Abstract：
In negative strand RNA viruses, ribonucleoproteins, not naked RNA, constitute the template used by the large protein endowed with polymerase activity for replicating and transcribing the viral genome. Here we give an overview of the structures and functions of the ribonucleoprotein from phleboviruses. The nucleocapsid monomer, which constitutes the basic structural unit, possesses a flexible arm allowing for a conformational switch between a closed monomeric state and the formation of a polymeric filamentous structure competent for viral RNA binding and encapsidation in the open state of N. The modes of N-N oligomerization as well as interactions with vRNA are described. Finally, recent advances in tomography open exciting perspectives for a more complete understanding of N-L interactions and the design of specific antiviral compounds.
摘要：
在负链RNA病毒中，核糖核蛋白,不是裸露的RNA,构成具有聚合酶活性的大蛋白质用于复制和转录病毒基因组的模板。在这里，我们概述了来自静脉病毒的核糖核蛋白的结构和功能。核衣壳单体，构成基本结构单元，具有柔性臂，允许在闭合单体状态和形成聚合丝状结构之间进行构象转换，该结构可在N的开放状态下进行病毒RNA结合和衣壳化。描述了N-N寡聚化模式以及与vRNA的相互作用。最后,层析成像的最新进展为更全面地了解N-L相互作用和特定抗病毒化合物的设计开辟了令人兴奋的前景。
