Abstract：
Rift Valley fever virus (RVFV) is an arthropod-borne virus (arbovirus) responsible for a severe zoonotic disease affecting a wide range of domestic and wild ruminants as well as humans. RVFV is endemic in many African countries and has also caused outbreaks in Madagascar and Arabian Peninsula. With regard to its wide geographical distribution, its potential to emerge in a new area, and its capability to trigger major health and economic crisis, it is essential to study and better understand several aspects of its life cycle and, in particular, its interactions with mammalian hosts and arthropod vectors. To do so, it is key for researchers to be able to amplify in vitro viral strains isolated from the field and determine accurately the viral titers of RVFV stocks. In this chapter, we present protocols that can be easily implemented to produce and titrate RVFV stocks in your laboratory.
摘要：
裂谷热病毒（RVFV）是一种节肢动物传播的病毒（虫媒病毒），可引起严重的人畜共患疾病，影响各种家畜和野生反刍动物以及人类。RVFV在许多非洲国家是地方病，也在马达加斯加和阿拉伯半岛引起了疫情。关于其广泛的地理分布，它在一个新领域出现的潜力，以及它引发重大健康和经济危机的能力，研究和更好地理解其生命周期的几个方面是至关重要的，特别是,它与哺乳动物宿主和节肢动物载体的相互作用。要做到这一点，研究人员能够扩增从该领域分离的体外病毒株并准确确定RVFV库存的病毒滴度是关键。在这一章中,我们提出的协议，可以很容易地实现生产和滴定RVFV股票在你的实验室。
