METHODS: A 50 μl Hamilton syringe attached to a disposable needle was percutaneously inserted into the subarachnoid space (cisterna magna) between the occipital bone and C1 lamina in an anesthetized adult mouse, followed by the injection of contrast medium and CT imaging.
RESULTS: CTM clearly visualized the shape of the spinal cord of intact mice and tiptoe-walking Yoshimura (Twy) mice without any health issues.
CONCLUSIONS: Unlike histology, the current method functions in live mice, directly depicts the compressed spinal cord, and provides clinically related image information. Furthermore, the intrathecal administration of contrast medium through the percutaneous route makes CTM less invasive and takes less time than a conventional intrathecal injection method.
CONCLUSIONS: The CTM method used in the present study enables clear visualization of the shape of the dural sac and spinal cord and is useful when conducting experiments on CCM and other spinal diseases in rodents.
方法:将固定在一次性针头上的50μlHamilton注射器经皮插入麻醉成年小鼠的枕骨和C1椎板之间的蛛网膜下腔(小脑池),然后注射造影剂和CT成像。
结果:CTM清楚地显示了完整小鼠和脚尖行走的吉村(Twy)小鼠的脊髓形状,没有任何健康问题。
结论:与组织学不同,目前的方法在活小鼠中起作用,直接描绘了压缩的脊髓,并提供临床相关图像信息。此外,与传统的鞘内注射方法相比,通过经皮途径鞘内注射造影剂使CTM的侵入性更小,花费的时间更少。
结论:本研究中使用的CTM方法可以清晰地显示硬膜囊和脊髓的形状,并且在进行啮齿动物的CCM和其他脊柱疾病的实验时很有用。