Abstract：
The role of adenosine receptors in fascial manipulation-induced analgesia has not yet been investigated. The purpose of this study was to evaluate the involvement of the adenosine A1 receptor (A1R) in the antihyperalgesic effect of plantar fascia manipulation (PFM), specifically in mice with peripheral inflammation. Mice injected with Complete Freund\'s Adjuvant (CFA) underwent behavioral, i.e. mechanical hyperalgesia and edema. The mice underwent PFM for either 3, 9 or 15 min. Response frequency to mechanical stimuli was then assessed at 24 and 96 h after plantar CFA injection. The adenosinergic receptors were assessed by systemic (intraperitoneal, i.p.), central (intrathecal, i.t.), and peripheral (intraplantar, i.pl.) administration of caffeine. The participation of the A1R was investigated using the 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), a selective A1R subtype antagonist. PFM inhibited mechanical hyperalgesia induced by CFA injection and did not reduce paw edema. Furthermore, the antihyperalgesic effect of PFM was prevented by pretreatment of the animals with caffeine given by i.p., i.pl., and i.t. routes. In addition, i.pl. and i.t. administrations of DPCPX blocked the antihyperalgesia caused by PFM. These observations indicate that adenosine receptors mediate the antihyperalgesic effect of PFM. Caffeine\'s inhibition of PFM-induced antihyperalgesia suggests that a more precise understanding of how fascia-manipulation and caffeine interact is warranted.
摘要：
尚未研究腺苷受体在筋膜操作诱导的镇痛中的作用。本研究的目的是评估腺苷A1受体（A1R）在足底筋膜操纵（PFM）的抗痛觉过敏作用中的参与，特别是在患有外周炎症的小鼠中。注射完全弗氏佐剂（CFA）的小鼠进行行为，即机械性痛觉过敏和水肿。小鼠接受PFM3、9或15分钟。然后在足底CFA注射后24和96小时评估对机械刺激的响应频率。通过全身性（腹膜内，i.p.),中央（鞘内，i.t.),和外周(足底内，i.pl.)咖啡因的管理。使用1,3-二丙基-8-环戊基黄嘌呤（DPCPX）研究了A1R的参与，选择性A1R亚型拮抗剂。PFM抑制了CFA注射引起的机械性痛觉过敏，并且不能减轻爪水肿。此外，PFM的抗痛觉过敏作用是通过用静脉注射的咖啡因对动物进行预处理来防止的。i.pl.,和i.t.路线。此外,i.pl.和i.t.DPCPX的给药阻断了由PFM引起的抗痛觉过敏。这些观察表明腺苷受体介导PFM的抗痛觉过敏作用。咖啡因对PFM诱导的抗痛觉过敏的抑制作用表明，有必要对筋膜操纵和咖啡因如何相互作用进行更精确的理解。
