Abstract：
Hidradenitis suppurativa (HS) is a chronic inflammatory disease that is difficult to control, and its mechanism remains unclear. Hepatocyte GF (HGF) has been reported to be significantly upregulated in the serum and skin of patients with HS, especially in the lesions with tunnels. In this study, we examined the transcriptome of HGF-treated keratinocytes and compared it with genetic profiling of HS lesions. HGF was highly expressed in HS skin, especially in the deep dermis, compared with that in healthy controls, and its source was mainly fibroblasts. HGF upregulated more genes in keratinocytes than IL-17A or TNF-a, and these genes included multiple epithelial-mesenchymal transition-related genes. Differentially expressed genes in HGF-stimulated keratinocytes were involved in activation of epithelial-mesenchymal transition-related pathways. These HGF-induced genes were significantly upregulated in HS lesions compared with those in healthy skin and nonlesions and were more strongly associated with HS tunnels. In summary, HGF was highly expressed in HS and induced epithelial-mesenchymal transition-related genes in keratinocytes; HGF-induced genes were highly associated with gene profiling of HS with tunnels, suggesting that HGF may be involved in HS tunnel formation through epithelial-mesenchymal transition.
摘要：
化脓性汗腺炎（HS）是一种难以控制的慢性炎症性疾病，其机制尚不清楚。肝细胞生长因子(HGF)已被报道在HS患者的血清和皮肤中显著上调,特别是在有隧道的病变中。在这项研究中,我们检查了HGF处理的角质形成细胞(KCs)的转录组,并将其与HS病变的遗传谱进行了比较.HGF在HS皮肤中高表达，尤其是在真皮深处,与健康对照相比，其来源主要是成纤维细胞。与白细胞介素-17A或肿瘤坏死因子-α相比，HGF在KCs中上调更多的基因，这些基因包括多个上皮间质转化（EMT）相关基因。HGF刺激的KC中差异表达的基因参与了EMT相关途径的激活。与健康皮肤和非损伤相比,这些HGF诱导的基因在HS损伤中显著上调,并且与HS隧道更强烈相关。总之,HGF在HS中高表达，并在KCs中诱导EMT相关基因；HGF诱导的基因与带隧道的HS基因谱高度相关，提示HGF可能通过EMT参与HS隧道的形成。
