Abstract：
Spodoptera frugiperda is a significant global pest, and chlorantraniliprole (CAP) is extensively used in China for its control. Understanding CAP resistance in S. frugiperda is crucial for effective management of this pest. Field populations exhibited varying degrees of resistance to CAP (RR = 1.74-5.60-fold). After 10 generations of selection, the CAP-resistant strain developed over 10-fold resistance, with a realized heritability (h2) of 0.10. Genetic analysis reveals inheritance patterns as autosomal, incomplete recessive, and monofactorial. The CAP-resistant strain showed limited cross-resistance to lufenuron and tetrachlorantraniliprole, negative cross-resistance to spinetoram, and no observed cross-resistance to other insecticides. Biochemical analysis suggested that P450-mediated detoxification is the primary resistance mechanism, with 26 genes overexpressed in the CAP-resistant strain. Additionally, the knockdown of CYP4L13, CYP6B39, CYP6B40, and CYP4G74 significantly increased the sensitivity of the resistant larvae to CAP. These findings highlight the resistance risk of CAP in S. frugiperda and emphasize the crucial role of P450 enzymes in resistance.
摘要：
节食夜蛾是一种重要的全球性害虫，并且在中国广泛使用chloantraniliprole（CAP）进行控制。了解S.frugiperda的CAP抗性对于有效管理这种害虫至关重要。田间种群对CAP表现出不同程度的抗性（RR=1.74-5.60倍）。经过10代的选择，抗CAP菌株发展超过10倍的抗性，实现遗传力(h2)为0.10。遗传分析揭示了遗传模式为常染色体，不完全隐性,和单因子。CAP抗性菌株对卢芬隆和四氯苯三烯的交叉抗性有限，对spinetoram的负交叉抗性，并且没有观察到对其他杀虫剂的交叉抗性。生化分析表明，P450介导的解毒是主要的耐药机制，26个基因在CAP抗性菌株中过表达。此外,CYP4L13，CYP6B39，CYP6B40和CYP4G74的敲除显着增加了抗性幼虫对CAP的敏感性。这些发现强调了在S.frugiperda中CAP的抗性风险，并强调了P450酶在抗性中的关键作用。
