Abstract：
Surface modification could enhance the cell internalization efficiency of nanovehicles for targeted gene or drug delivery. However, the influence of surface modification parameters, including recognition manners, valences, and patterns, is often clouded, especially for the endocytosis of DNA nanostructures in customized shapes. Focusing on an icosahedral DNA framework, we systematically programmed three distinct types of ligands with diverse valence and spatial distribution on their outer surface to study the internalization efficiency, endocytic pathways, and postinternalization fate. The comparison in different aspects of parameters deepens our understanding of the intricate relationship between surface modification and cell entry behavior, offering insights crucial for designing and optimizing DNA framework nanostructures for potent cell-targeted purposes.
摘要：
表面修饰可以增强用于靶向基因或药物递送的纳米载体的细胞内化效率。然而,表面改性参数的影响，包括认可方式，化合价,和模式，经常阴云密布，特别是对于定制形状的DNA纳米结构的内吞作用。专注于二十面体DNA框架，我们系统地编程了三种不同类型的配体，它们的外表面具有不同的价态和空间分布，以研究内化效率，胞吞途径，和内化后的命运。参数不同方面的比较加深了我们对表面修饰和细胞进入行为之间复杂关系的理解。为有效的细胞靶向目的提供设计和优化DNA框架纳米结构至关重要的见解。
