Abstract：
Here, we describe a clinically relevant xenograft model of hormone receptor-positive breast cancer that maintains estrogen receptor (ER) status without the need for exogenous supplementation of hormones. The naturally low 17-β-estradiol levels in host mice recapitulate levels seen in post-menopausal women. By introducing breast cancer cells directly into their \"natural\" microenvironment of the milk ducts, these cells maintain hormone receptor status, model the clinical progression of the disease, and develop ER- metastatic lesions or dormant micrometastatic lesions in the case of ER+ BC. With the use of GFP/RFP:Luc2 reporters, we can monitor in vivo tumour growth and conduct ex vivo metastases assays to evaluate dormant metastatic cell harboring organs. Upon recovery of metastatic cells from ER+ breast cancer models, downstream analyses can be conducted to assess the relationship between epithelial plasticity and metastatic dormancy.
摘要：
这里,我们描述了激素受体阳性乳腺癌的临床相关异种移植模型,该模型无需外源性补充激素即可维持雌激素受体(ER)状态.宿主小鼠中自然较低的17-β-雌二醇水平概括了绝经后妇女中的水平。通过将乳腺癌细胞直接引入乳管的“天然”微环境，这些细胞维持激素受体状态，模拟疾病的临床进展，并在ERBC的情况下发展为ER-转移性病变或休眠微转移性病变。使用GFP/RFP：Luc2报告分子，我们可以监测体内肿瘤的生长，并进行离体转移试验，以评估休眠的转移细胞携带器官。从ER+乳腺癌模型中恢复转移细胞后，可以进行下游分析以评估上皮可塑性和转移性休眠之间的关系.
