Abstract：
In our study, a method based on affinity ultrafiltration screening coupled with UPLC-ESI-Orbitrap-MS technology was established to select Glucagon-like peptide-1 receptor (GLP-1R) agonists from natural products, and as an example, the GLP-1R agonists from Panax ginseng was selected using our established method. As a result, total five GLP-1R agonists were selected from Panax ginseng for the first time. Our results indicated that activating GLP-1R to promote insulin secretion probably was another important hypoglycemia mechanism for ginsenosides in Panax ginseng, which had great influence on the study of the anti-diabetes effect of ginsenosides.
摘要：
在我们的研究中,建立了基于亲和超滤筛选结合UPLC-ESI-Orbitrap-MS技术从天然产物中筛选胰高血糖素样肽-1受体(GLP-1R)激动剂的方法,作为一个例子，使用我们建立的方法从人参中选择GLP-1R激动剂.因此,首次从人参中筛选出5种GLP-1R激动剂。我们的结果表明，激活GLP-1R促进胰岛素分泌可能是人参中人参皂苷的另一个重要低血糖机制。对人参皂苷抗糖尿病作用的研究有很大影响。
