Abstract：
Mesenchymal stem cells (MSCs) are a population of multipotent cells with remarkable regenerative and immunomodulatory properties. Wharton\'s jelly (WJ) from the umbilical cord (UC) has gained increasing interest in the biomedical field as an outstanding source of MSCs. However, challenges such as limited supply and lack of standardization in existing methods have arisen. This article presents a novel method for enhancing MSC yield by dissecting intact WJ from the umbilical cord. The method employs blunt dissection to remove the epithelial layer, maintaining the integrity of the entire WJ and resulting in an increased quantity and viability of harvested MSCs. This approach significantly reduces WJ waste compared to conventional sharp dissection methods. To ensure the purity of WJ-MSCs and minimize external cellular influence, a procedure utilizing internal tension to peel off the endothelium after flipping the UC was conducted. Additionally, the Petri dish was inverted for a short time during explant culture to improve attachment and cell outgrowth. Comparative analysis demonstrated the superiority of the proposed method, showing a higher yield of WJ and WJ-MSCs with better viability than traditional methods. The similar morphology and expression pattern of cell surface markers in both methods confirm their characterization and purity for various applications. This method provides a high-yield and high-viability approach for WJ-MSC isolation, demonstrating great potential for the clinical application of MSCs.
摘要：
间充质干细胞（MSC）是具有显着的再生和免疫调节特性的多能细胞群体。脐带（UC）的Wharton果冻（WJ）作为MSCs的杰出来源在生物医学领域获得了越来越多的兴趣。然而,出现了供应有限和现有方法缺乏标准化等挑战。本文提出了一种通过从脐带中解剖完整的WJ来提高MSC产量的新方法。该方法采用钝性解剖来去除上皮层，维持整个WJ的完整性并导致收获的MSC的数量和活力增加。与传统的尖锐解剖方法相比，这种方法显着减少了WJ浪费。为了确保WJ-MSCs的纯度并将外部细胞影响降至最低，在翻转UC后,进行利用内部张力剥离内皮的程序。此外,在外植体培养过程中短时间倒置培养皿以改善附着和细胞生长。对比分析证明了该方法的优越性,显示出比传统方法具有更好活力的WJ和WJ-MSC的更高产量。两种方法中细胞表面标志物的相似形态和表达模式证实了它们在各种应用中的表征和纯度。该方法为WJ-MSC的分离提供了一种高产量和高活力的方法，证明了MSCs临床应用的巨大潜力。
