Abstract：
Catheter-associated urinary tract infection (CAUTI) is the most common healthcare-associated infection; however, current therapeutic strategies remain insufficient for standard clinical application. A novel urinary catheter featuring a dual-layer nanoengineering approach using zinc (Zn) and silver nanoparticles (AgNPs) is successfully fabricated. This design targets microbial resistance, minimizes cytotoxicity, and maintains long-term efficacy. The inner AgNPs layer provides immediate antibacterial effects against the UTI pathogens, while the outer porous Zn layer controls zero-order Ag release and generates reactive oxygen species, thus enhancing long-term bactericidal performance. Enhanced antibacterial properties of Zn/AgNPs-coated catheters are observed, resulting in 99.9% of E. coli and 99.7% of S. aureus reduction, respectively. The Zn/AgNPs-coated catheter significantly suppresses biofilm with sludge formation compared to AgNP-coated and uncoated catheters (all, p < 0.05). The Zn/AgNP-coated catheter in a rabbit model demonstrated a durable, effective barrier against bacterial colonization, maintaining antimicrobial properties during the catheter indwelling period with significantly reduced inflammation and epithelial disruption compared with AgNP and uncoated groups. This innovation has the potential to revolutionize the design of antimicrobial medical devices, particularly for applications requiring long-term implantation. Although further preclinical studies are required to verify its efficacy and safety, this strategy seems to be a promising approach to preventing CAUTI-related complications.
摘要：
导管相关尿路感染(CAUTI)是最常见的医疗保健相关感染;然而,目前的治疗策略仍然不足以用于标准的临床应用.成功制造了一种新型导尿管，该导尿管具有使用锌（Zn）和银纳米颗粒（AgNPs）的双层纳米工程方法。这种设计针对微生物抗性，最小化细胞毒性，并保持长期疗效。内部AgNPs层提供对UTI病原体的即时抗菌作用，而外部多孔Zn层控制零级Ag释放并产生活性氧，从而提高长期杀菌性能。观察到Zn/AgNPs涂层导管的抗菌性能增强，导致99.9%的大肠杆菌和99.7%的金黄色葡萄球菌减少，分别。与AgNP涂层和未涂层的导管相比，Zn/AgNP涂层的导管显着抑制了污泥形成的生物膜（所有，p<0.05)。在兔子模型中，Zn/AgNP涂层的导管表现出耐用，防止细菌定植的有效屏障，与AgNP和未涂层组相比，在导管留置期间保持抗菌性能，并显着减少炎症和上皮破坏。这项创新有可能彻底改变抗菌医疗设备的设计，特别是需要长期植入的应用。尽管需要进一步的临床前研究来验证其有效性和安全性，这一策略似乎是预防CAUTI相关并发症的有希望的方法.
