PDF(Pubmed)
Abstract：
UNASSIGNED: Herbal components, particularly sesquiterpenes, are progressively recognized as a crucial resource for developing effective therapeutic agents for breast cancer. In this study, the effect of a sesquiterpene lactone known as 8-O-dihydroxy-11a,13-dihydroeudesma-4(15)-en-12,6a-olide (persianolide- A) was examined in breast cancer cell lines.
UNASSIGNED: MDA-MB-231 and MCF-7 cancer cells were grown in DMEM solution with 10% FBS. Then, an MTT assay was performed to evaluate cell viability. Apoptosis was detected by annexin-PI staining. A caspase 3/7 activity assay kit was used to assess the activity of caspase-3 and caspase-7. Protein expression of Bcl-2, Bax, and p-ERK1/2 was determined by western blotting.
UNASSIGNED: This study showed that the IC50 values of the persianolide-A for MCF-7 and MDA-MB- 468 cells are 34.76 and 54.48 μM, respectively. In addition, persianolide-A showed a significant increase in apoptosis in both MDAMB-231 and MCF-7 breast cancer cell lines. Persianolide-A significantly increased the expression of the pro-apoptotic protein Bax and decreased the expression of the anti-apoptotic protein Bcl-2. Also, presinolide-A treatment led to a substantial increase in caspase activity with a ratio of 3/7 in both MCF- 7 and MDA-MB-231 cancer cells. In addition, the study showed that persianolide-A decreased the expression of p-ERK1/2 protein.
UNASSIGNED: The results of this study suggest that persianolide-A, sourced from Artemisia kopetdaghensis, induces cell apoptosis in breast cancer cell types. The molecular mechanisms could be implicated in the modulation of the ERK1/2 signaling pathway.
UNASSIGNED: MDA-MB-231 and MCF-7 cancer cells were grown in DMEM solution with 10% FBS. Then, an MTT assay was performed to evaluate cell viability. Apoptosis was detected by annexin-PI staining. A caspase 3/7 activity assay kit was used to assess the activity of caspase-3 and caspase-7. Protein expression of Bcl-2, Bax, and p-ERK1/2 was determined by western blotting.
UNASSIGNED: This study showed that the IC50 values of the persianolide-A for MCF-7 and MDA-MB- 468 cells are 34.76 and 54.48 μM, respectively. In addition, persianolide-A showed a significant increase in apoptosis in both MDAMB-231 and MCF-7 breast cancer cell lines. Persianolide-A significantly increased the expression of the pro-apoptotic protein Bax and decreased the expression of the anti-apoptotic protein Bcl-2. Also, presinolide-A treatment led to a substantial increase in caspase activity with a ratio of 3/7 in both MCF- 7 and MDA-MB-231 cancer cells. In addition, the study showed that persianolide-A decreased the expression of p-ERK1/2 protein.
UNASSIGNED: The results of this study suggest that persianolide-A, sourced from Artemisia kopetdaghensis, induces cell apoptosis in breast cancer cell types. The molecular mechanisms could be implicated in the modulation of the ERK1/2 signaling pathway.
摘要：
■草药成分，尤其是倍半萜,逐渐被认为是开发乳腺癌有效治疗剂的关键资源。在这项研究中,称为8-O-二羟基-11a的倍半萜内酯的作用，在乳腺癌细胞系中检查了13-二氢eudesma-4（15）-en-12,6a-olide（persianolide-A）。
■MDA-MB-231和MCF-7癌细胞在含有10%FBS的DMEM溶液中生长。然后，进行MTT测定以评估细胞活力。通过膜联蛋白-PI染色检测细胞凋亡。使用半胱天冬酶3/7活性测定试剂盒来评估半胱天冬酶-3和半胱天冬酶-7的活性。Bcl-2、Bax、和p-ERK1/2通过蛋白质印迹法测定。
■这项研究表明，persianolide-A对MCF-7和MDA-MB-468细胞的IC50值分别为34.76和54.48μM，分别。此外,persianolide-A在MDAMB-231和MCF-7乳腺癌细胞系中均显示出细胞凋亡的显着增加。Persianolide-A显着增加促凋亡蛋白Bax的表达，并降低抗凋亡蛋白Bcl-2的表达。此外，在MCF-7和MDA-MB-231癌细胞中,前诺利-A处理导致半胱天冬酶活性的显著增加,比例为3/7。此外,研究表明，persianolide-A降低了p-ERK1/2蛋白的表达。
■这项研究的结果表明，persianolide-A，来自kopetdaghensis蒿，诱导乳腺癌细胞类型的细胞凋亡。分子机制可能与ERK1/2信号通路的调节有关。
■MDA-MB-231和MCF-7癌细胞在含有10%FBS的DMEM溶液中生长。然后，进行MTT测定以评估细胞活力。通过膜联蛋白-PI染色检测细胞凋亡。使用半胱天冬酶3/7活性测定试剂盒来评估半胱天冬酶-3和半胱天冬酶-7的活性。Bcl-2、Bax、和p-ERK1/2通过蛋白质印迹法测定。
■这项研究表明，persianolide-A对MCF-7和MDA-MB-468细胞的IC50值分别为34.76和54.48μM，分别。此外,persianolide-A在MDAMB-231和MCF-7乳腺癌细胞系中均显示出细胞凋亡的显着增加。Persianolide-A显着增加促凋亡蛋白Bax的表达，并降低抗凋亡蛋白Bcl-2的表达。此外，在MCF-7和MDA-MB-231癌细胞中,前诺利-A处理导致半胱天冬酶活性的显著增加,比例为3/7。此外,研究表明，persianolide-A降低了p-ERK1/2蛋白的表达。
■这项研究的结果表明，persianolide-A，来自kopetdaghensis蒿，诱导乳腺癌细胞类型的细胞凋亡。分子机制可能与ERK1/2信号通路的调节有关。
