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Abstract：
The Traditional Chinese Medicine compound preparation known as Diwu Yanggan capsule (DWYG) can effectively hinder the onset and progression of hepatocellular carcinoma (HCC), which is recognized worldwide as a significant contributor to fatalities associated with cancer. Nevertheless, the precise mechanisms implicated have remained ambiguous. In present study, the model of HCC was set up by the 2-acetylaminofluorene (2-AAF)/partial hepatectomy (PH) in rats. To confirm the differentially expressed genes (DEGs) identified in the microarray analysis, real-time quantitative reverse transcription PCR (qRT-PCR) was conducted. In the meantime, the liquid chromatography-quadrupole time of flight mass spectrometry (LC-QTOF-MS/MS) was employed to characterize the component profile of DWYG. Consequently, the DWYG treatment exhibited the ability to reverse 51 variation genes induced by 2-AAF/PH. Additionally, there was an overlap of 54 variation genes between the normal and model groups. Upon conducting RT-qPCR analysis, it was observed that the expression levels of all genes were increased by 2-AAF/PH and subsequently reversed after DWYG treatment. Notably, the fold change of expression levels for all genes was below 0.5, with 3 genes falling below 0.25. Moreover, an investigation was conducted to determine the signaling pathway that was activated/inhibited in the HCC group and subsequently reversed in the DWYG group. Moreover, the component profile of DWYG encompassed a comprehensive compilation of 206 compounds that were identified or characterized. The findings of this study elucidated the potential alleviative mechanisms of DWYG in the context of HCC, thereby holding significant implications for its future clinical utilization and widespread adoption.
摘要：
中药复方制剂地物养肝胶囊(DWYG)能有效阻碍肝细胞癌(HCC)的发生发展,这在世界范围内被认为是与癌症相关的死亡的重要原因。然而，所涉及的确切机制仍然模棱两可。在目前的研究中,2-乙酰氨基芴（2-AAF）/部分肝切除术（PH）建立大鼠HCC模型。为了确认在微阵列分析中鉴定的差异表达基因(DEGs),实时定量逆转录PCR(qRT-PCR)。同时,采用液相色谱-四极杆飞行时间质谱（LC-QTOF-MS/MS）表征DWYG的组分图谱。因此,DWYG处理显示出逆转2-AAF/PH诱导的51个变异基因的能力。此外,正常组和模型组之间有54个变异基因重叠。在进行RT-qPCR分析后，观察到所有基因的表达水平被2-AAF/PH增加,随后在DWYG处理后逆转。值得注意的是,所有基因表达水平的倍数变化均低于0.5，其中3个基因低于0.25。此外，进行了一项研究,以确定在HCC组中被激活/抑制,随后在DWYG组中被逆转的信号通路.此外，DWYG的成分概况包括对已鉴定或表征的206种化合物的全面汇编.这项研究的结果阐明了DWYG在HCC中的潜在缓解机制，从而对其未来的临床应用和广泛采用具有重要意义。
