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Abstract：
UNASSIGNED: In the field of conservative dentistry and endodontics, mineral trioxide aggregate (MTA), commonly used, possesses advantages such as biocompatibility, antimicrobial properties and osteogenic potential. This study investigated the feasibility of utilizing membrane form mineral trioxide aggregate (MTA) as a barrier membrane in guided bone regeneration (GBR) procedures.
UNASSIGNED: Membranes were electrospun from three different formulations: 15 w/v% Polycaprolactone (PCL), 13 w/v% PCL + 2 w/v% MTA (2MTA), and 11 w/v% PCL + 4 w/v% MTA (4MTA). Physicochemical and mechanical properties of the electrospun membrane were compared, encompassing parameters such as surface morphology, fiber diameter distribution, chemical composition, phase identification, tensile stress, pH variation, and water contact angle. Moreover, the antimicrobial properties against of the electrospun membranes were assessed through direct exposure to streptococcus aureus (S. aureus) and candida albicans (C. albicans). Additionally, on the 7th day, biocompatibility and cell attachment were investigated with respect to L929 (fibroblast) and MC3T3 (pre-osteoblast) cells. Inhibition of L929 cell infiltration and the expression of osteogenic related genes including osteocalcin (OCN), alkaline phosphatase (ALP), and runt related transcription factor 2 (RUNX2) in MC3T3 cells on 7th and 14th days were also investigated.
UNASSIGNED: PCL, 2MTA, and 4MTA exhibited no statistically differences in fiber diameter distribution and tensile stress. However, as the MTA content increased, wettability and pH also increased. Due to the elevated pH, 4MTA demonstrated the lowest viability S.aureus and C.albicans. All membranes were highly biocompatibility and promoted cell attachment, while effectively preventing L929 cell infiltration. Lastly 4MTA showed increase in OCN, ALP, and RUNX2 expression on both 7th and 14th day.
UNASSIGNED: The membrane form MTA possessed characteristics essential for a novel barrier membrane.
UNASSIGNED: Membranes were electrospun from three different formulations: 15 w/v% Polycaprolactone (PCL), 13 w/v% PCL + 2 w/v% MTA (2MTA), and 11 w/v% PCL + 4 w/v% MTA (4MTA). Physicochemical and mechanical properties of the electrospun membrane were compared, encompassing parameters such as surface morphology, fiber diameter distribution, chemical composition, phase identification, tensile stress, pH variation, and water contact angle. Moreover, the antimicrobial properties against of the electrospun membranes were assessed through direct exposure to streptococcus aureus (S. aureus) and candida albicans (C. albicans). Additionally, on the 7th day, biocompatibility and cell attachment were investigated with respect to L929 (fibroblast) and MC3T3 (pre-osteoblast) cells. Inhibition of L929 cell infiltration and the expression of osteogenic related genes including osteocalcin (OCN), alkaline phosphatase (ALP), and runt related transcription factor 2 (RUNX2) in MC3T3 cells on 7th and 14th days were also investigated.
UNASSIGNED: PCL, 2MTA, and 4MTA exhibited no statistically differences in fiber diameter distribution and tensile stress. However, as the MTA content increased, wettability and pH also increased. Due to the elevated pH, 4MTA demonstrated the lowest viability S.aureus and C.albicans. All membranes were highly biocompatibility and promoted cell attachment, while effectively preventing L929 cell infiltration. Lastly 4MTA showed increase in OCN, ALP, and RUNX2 expression on both 7th and 14th day.
UNASSIGNED: The membrane form MTA possessed characteristics essential for a novel barrier membrane.
摘要：
■在保守牙科和牙髓学领域，矿物三氧化物骨料（MTA），常用的,具有生物相容性等优点，抗菌性能和成骨潜力。这项研究调查了在引导骨再生（GBR）程序中利用膜形式的三氧化物矿物聚集体（MTA）作为屏障膜的可行性。
■从三种不同的配方静电纺丝膜：15w/v%聚己内酯（PCL），13w/v%PCL+2w/v%MTA(2MTA)，和11w/v%PCL+4w/v%MTA(4MTA)。比较了静电纺丝膜的物理化学和机械性能，包括表面形态等参数，纤维直径分布，化学成分,相位识别，拉伸应力,pH变化,和水接触角。此外，通过直接暴露于金黄色链球菌（S.金黄色葡萄球菌)和白色念珠菌(C.albicans).此外,第七天,研究了L929（成纤维细胞）和MC3T3（前成骨细胞）细胞的生物相容性和细胞附着。抑制L929细胞浸润和骨钙蛋白（OCN）成骨相关基因的表达，碱性磷酸酶(ALP),还研究了第7天和第14天MC3T3细胞中的runt相关转录因子2（RUNX2）。
■PCL,2MTA,和4MTA在纤维直径分布和拉伸应力方面没有统计学差异。然而,随着MTA含量的增加，润湿性和pH也增加。由于pH值升高,4MTA表现出最低的活力金黄色葡萄球菌和白色念珠菌。所有的膜都是高度生物相容性和促进细胞附着，同时有效防止L929细胞浸润。最后，4MTA显示OCN增加，ALP,和RUNX2表达在第7天和第14天。
■膜形式MTA具有新型屏障膜所必需的特性。
■从三种不同的配方静电纺丝膜：15w/v%聚己内酯（PCL），13w/v%PCL+2w/v%MTA(2MTA)，和11w/v%PCL+4w/v%MTA(4MTA)。比较了静电纺丝膜的物理化学和机械性能，包括表面形态等参数，纤维直径分布，化学成分,相位识别，拉伸应力,pH变化,和水接触角。此外，通过直接暴露于金黄色链球菌（S.金黄色葡萄球菌)和白色念珠菌(C.albicans).此外,第七天,研究了L929（成纤维细胞）和MC3T3（前成骨细胞）细胞的生物相容性和细胞附着。抑制L929细胞浸润和骨钙蛋白（OCN）成骨相关基因的表达，碱性磷酸酶(ALP),还研究了第7天和第14天MC3T3细胞中的runt相关转录因子2（RUNX2）。
■PCL,2MTA,和4MTA在纤维直径分布和拉伸应力方面没有统计学差异。然而,随着MTA含量的增加，润湿性和pH也增加。由于pH值升高,4MTA表现出最低的活力金黄色葡萄球菌和白色念珠菌。所有的膜都是高度生物相容性和促进细胞附着，同时有效防止L929细胞浸润。最后，4MTA显示OCN增加，ALP,和RUNX2表达在第7天和第14天。
■膜形式MTA具有新型屏障膜所必需的特性。
