PDF(Pubmed)
Abstract：
Mango is a popular tropical fruit that requires quarantine hot water treatment (QHWT) for postharvest sanitation, which can cause abiotic stress. Plants have various defense mechanisms to cope with stress; miRNAs mainly regulate the expression of these defense responses. Proteins involved in the biogenesis of miRNAs include DICER-like (DCL), ARGONAUTE (AGO), HYPONASTIC LEAVES 1 (HYL1), SERRATE (SE), HUA ENHANCER1 (HEN1), HASTY (HST), and HEAT-SHOCK PROTEIN 90 (HSP90), among others. According to our analysis, the mango genome contains five DCL, thirteen AGO, six HYL, two SE, one HEN1, one HST, and five putative HSP90 genes. Gene structure prediction and domain identification indicate that sequences contain key domains for their respective gene families, including the RNase III domain in DCL and PAZ and PIWI domains for AGOs. In addition, phylogenetic analysis indicates the formation of clades that include the mango sequences and their respective orthologs in other flowering plant species, supporting the idea these are functional orthologs. The analysis of cis-regulatory elements of these genes allowed the identification of MYB, ABRE, GARE, MYC, and MeJA-responsive elements involved in stress responses. Gene expression analysis showed that most genes are induced between 3 to 6 h after QHWT, supporting the early role of miRNAs in stress response. Interestingly, our results suggest that mango rapidly induces the production of miRNAs after heat stress. This research will enable us to investigate further the regulation of gene expression and its effects on commercially cultivated fruits, such as mango, while maintaining sanitary standards.
摘要：
芒果是一种流行的热带水果，需要检疫热水处理（QHWT）进行采后卫生，会引起非生物胁迫。植物有多种防御机制来应对胁迫;miRNAs主要调节这些防御反应的表达。参与miRNA生物发生的蛋白质包括DICER样（DCL），ARGONAUTE(AGO),垂体叶1（HYL1），SERRATE(SE),HUAENHANCER1(HEN1),HASTY(HST),和热休克蛋白90（HSP90），在其他人中。根据我们的分析,芒果基因组包含5个DCL,十三岁前，六个HYL,两个SE，一个HEN1，一个HST，和五个推定的HSP90基因。基因结构预测和结构域鉴定表明序列含有各自基因家族的关键结构域,包括DCL中的RNaseIII结构域以及AGO的PAZ和PIWI结构域。此外,系统发育分析表明，在其他开花植物物种中，包括芒果序列及其各自的直系同源物的进化枝的形成，支持这个想法，这些是功能直系同源物。这些基因的顺式调控元件的分析允许鉴定MYB，ABRE,GARE,MYC,和MeJA响应元素参与应激反应。基因表达分析表明，大多数基因在QHWT后3至6小时内被诱导，支持miRNA在应激反应中的早期作用。有趣的是,我们的结果表明，芒果在热应激后迅速诱导miRNAs的产生。这项研究将使我们进一步研究基因表达的调控及其对商业栽培水果的影响，比如芒果,同时保持卫生标准。
