Abstract：
Mysids are small crustaceans that are closely related to shrimp/prawns and crabs but not subject to food allergen labeling requirements for raw materials. In the past, a processed food that contained Japanese smelt (wakasagi) was suspected of producing a false-positive result in shrimp/prawn and crab allergen test because of the presence of consumed mysids. However, there was no reported methods to confirm mysid presence. Therefore, we developed a PCR method to detect mysids. The developed PCR method had high specificity for a mysid species, with no amplification observed from samples of shrimp, crab, krill, mantis shrimp, or the meat of Japanese smelt. In addition, DNA extracted from the internal organs of Japanese smelt was amplified by this PCR method, and sequencing revealed mysid DNA. This confirmed that mysids remained in the internal organs of Japanese smelt following consumption. This PCR method for mysid detection even amplified Japanese smelt-containing processed food samples that were suspected to have produced a false-positive result in shrimp/prawn and crab ELISA. Thus, this PCR method would enable to detect such false positives are caused by mysid contamination.
摘要：
Mysids是与虾/虾和螃蟹密切相关的小型甲壳类动物，但不受原料食品过敏原标签要求的限制。在过去,一种含有日本冶炼物（wakasagi）的加工食品被怀疑在虾/虾和蟹过敏原测试中产生假阳性结果，因为存在食用的霉菌。然而,没有报道的方法来确认mysid的存在.因此,我们开发了一种PCR方法来检测霉菌。所开发的PCR方法对一株菌类有很高的特异性,从虾样本中没有观察到扩增，螃蟹,磷虾,，,,,或者日本的肉。此外,通过这种PCR方法扩增了从日本冶炼内部器官中提取的DNA，测序显示了MysidDNA.这证实了食用后，日本冶炼的内脏器官中仍有霉菌。这种用于mysid检测的PCR方法甚至扩增了含有日本冶炼的加工食品样品，这些样品被怀疑在虾/虾和蟹ELISA中产生了假阳性结果。因此，这种PCR方法将能够检测这种假阳性是由霉菌污染引起的。
