Abstract：
Emu oil is the oil extracted from the body fat of the Australian bird emu. Although previous studies have reported that emu oil has anti-inflammatory effects, the effect and mechanism of emu oil on the treatment of atopic dermatitis have not been reported. Here, 2, 4-dinitrofluorobenzene was used to induce atopic dermatitis-like appearance on the back skin of C57BL/6 mice. And then, the effect of emu oil in the atopic dermatitis treatment was evaluated. We found that emu oil reduced the transdermal water loss in the atopic dermatitis model. Additionally, the epidermal thickness treated with emu oil was significantly thinner. The number of mast cells and inflammatory cells were significantly decreased. The thymic stromal lymphopoietin (TSLP), which is secreted by keratinocyte, was decreased significantly after treatment. Moreover, the serum levels of cytokines TSLP, interleukin-4, interleukin-13, and immunoglobulin (Ig) E were decreased after emu oil treatment. Surprisingly, we found that the high level of Cdc42 expression in the atopic dermatitis, which was decreased after emu oil treatment. To detect the role of Cdc42 in atopic dermatitis, we constructed atopic dermatitis model in mice with sustained activation of Cdc42 signaling. Furthermore, we have confirmed that emu oil demonstrates anti-inflammatory effects in atopic dermatitis by inhibiting the expression of Cdc42 signaling in keratinocytes. In conclusion, we discovered a new role of Cdc42 in the development of atopic dermatitis, which mediated the therapeutic effect of emu oil on atopic dermatitis.
摘要：
Emu油是从澳大利亚鸟类e的体内脂肪中提取的油。尽管之前的研究已经报道过，emu油具有抗炎作用，此外,在特应性皮炎的治疗中,尚未见报道。这里,2，4-二硝基氟苯用于在C57BL/6小鼠的背部皮肤上诱导特应性皮炎样出现。然后,评估了emu油在特应性皮炎治疗中的作用。我们发现，在特应性皮炎模型中，e油减少了透皮失水。此外,emu油处理的表皮厚度明显变薄。肥大细胞和炎症细胞的数量显著减少。胸腺基质淋巴细胞生成素(TSLP),它是由角质形成细胞分泌的，治疗后明显下降。此外，细胞因子TSLP的血清水平，在emu油处理后，白细胞介素4，白细胞介素13和免疫球蛋白（Ig）E降低。令人惊讶的是,我们发现在特应性皮炎中Cdc42的高水平表达，它在处理过emu油后下降了。为了检测Cdc42在特应性皮炎中的作用，我们构建了Cdc42信号持续激活的小鼠特应性皮炎模型。此外，我们已经证实，emu油通过抑制角质形成细胞中Cdc42信号的表达在特应性皮炎中表现出抗炎作用。总之,我们发现了Cdc42在特应性皮炎发展中的新作用，它介导了emu油对特应性皮炎的治疗作用。
