Abstract：
The Chinese hamster ovary (CHO) cell is an epithelial-like cell that produces proteins with post-translational modifications similar to human glycosylation. It is widely used in the production of recombinant therapeutic proteins and monoclonal antibodies. Culturing CHO cells typically requires the addition of a certain proportion of fetal bovine serum (FBS) to maintain cell proliferation and passaging. However, serum is characterized by its complex composition, batch-to-batch variability, high cost, and potential risk of exogenous contaminants such as mycoplasma and viruses, which impact the purity and safety of the synthesized proteins. Therefore, search for serum alternatives and development of serum-free media for CHO-based protein biomanufacturing are of great significance. This review systematically summarizes the application advantages of CHO cells and strategies for high-density expression. It highlights the developmental trends of serum substitutes from human platelet lysates to animal-free extracts and microbial-derived substances and elucidates the mechanisms by which these substitutes enhance CHO cell culture performance and recombinant protein production, aiming to provide theoretical guidance for exploring novel serum alternatives and developing serum-free media for CHO cells.
摘要：
中国仓鼠卵巢(CHO)细胞是上皮样细胞,其产生具有类似于人糖基化的翻译后修饰的蛋白质。它广泛用于生产重组治疗性蛋白质和单克隆抗体。培养CHO细胞通常需要添加一定比例的胎牛血清(FBS)以维持细胞增殖和传代。然而,血清的特点是其复杂的成分，批次间的可变性，高成本,和潜在的风险的外源性污染物，如支原体和病毒，影响合成蛋白质的纯度和安全性。因此,寻找血清替代品和开发基于CHO的蛋白质生物制造的无血清培养基具有重要意义。本文系统总结了CHO细胞的应用优势和高密度表达策略。它强调了从人血小板裂解物到无动物提取物和微生物来源物质的血清替代品的发展趋势，并阐明了这些替代品增强CHO细胞培养性能和重组蛋白生产的机制，旨在为探索新型血清替代品和开发CHO细胞无血清培养基提供理论指导。
