Abstract：
BACKGROUND: To explore the correlation between short-chain fatty acids (SCFAs) in the peri-implant sulcular fluid (PISF) and peri-implant diseases.
METHODS: PISF samples were obtained from implants that have been placed for at least 5 years, and peri-implant clinical parameters were examined. Gas chromatography-mass spectrometry and high-performance liquid chromatography were used to detect SCFAs in PISF. The correlation between SCFAs and clinical parameters was analyzed by Spearman\'s correlation. SCFAs related to peri-implant disease were identified by logistic regression and ranked by random forest analysis.
RESULTS: Eighty-six implants were divided into a peri-implant health group (PIH-group, 35 implants), peri-implant mucositis group (PIM-group, 25 implants), and peri-implantitis group (PI-group, 26 implants) according to clinical and radiographic examination results. The PIM-group had significantly lower formic acid detection rate than the other two groups (p < 0.001). The PIM-group had significantly higher levels of acetic, propionic, and isovaleric acids than the PIH-group (p < 0.05). The PI-group had significantly higher levels of propionic, butyric, isobutyric, valeric, and isovaleric acids than the PIH-group (p < 0.05). The PI-group had significantly higher levels of butyric, isobutyric, and isovaleric acids than the PIM-group (p < 0.05). SCFAs (apart from hexanoic and succinic acids) were significantly and positively correlated with clinical parameters (p < 0.05). SCFAs related to peri-implant disease were ranked as follows: butyric, isovaleric, isobutyric, propionic, acetic, formic, and lactic acids.
CONCLUSIONS: Elevated specific SCFAs are correlated with peri-implant disease. Recognition of this correlation may help in early identification of peri-implant disease and guide further clinical interventions.
METHODS: PISF samples were obtained from implants that have been placed for at least 5 years, and peri-implant clinical parameters were examined. Gas chromatography-mass spectrometry and high-performance liquid chromatography were used to detect SCFAs in PISF. The correlation between SCFAs and clinical parameters was analyzed by Spearman\'s correlation. SCFAs related to peri-implant disease were identified by logistic regression and ranked by random forest analysis.
RESULTS: Eighty-six implants were divided into a peri-implant health group (PIH-group, 35 implants), peri-implant mucositis group (PIM-group, 25 implants), and peri-implantitis group (PI-group, 26 implants) according to clinical and radiographic examination results. The PIM-group had significantly lower formic acid detection rate than the other two groups (p < 0.001). The PIM-group had significantly higher levels of acetic, propionic, and isovaleric acids than the PIH-group (p < 0.05). The PI-group had significantly higher levels of propionic, butyric, isobutyric, valeric, and isovaleric acids than the PIH-group (p < 0.05). The PI-group had significantly higher levels of butyric, isobutyric, and isovaleric acids than the PIM-group (p < 0.05). SCFAs (apart from hexanoic and succinic acids) were significantly and positively correlated with clinical parameters (p < 0.05). SCFAs related to peri-implant disease were ranked as follows: butyric, isovaleric, isobutyric, propionic, acetic, formic, and lactic acids.
CONCLUSIONS: Elevated specific SCFAs are correlated with peri-implant disease. Recognition of this correlation may help in early identification of peri-implant disease and guide further clinical interventions.
摘要：
背景：探讨种植体周围沟液（PISF）中的短链脂肪酸（SCFAs）与种植体周围疾病的相关性。
方法：从已放置至少5年的植入物中获得PISF样品，和种植体周围的临床参数进行了检查。采用气相色谱-质谱法和高效液相色谱法检测PISF中的SCFA。采用Spearman相关性分析SCFAs与临床参数的相关性。与种植体周围疾病相关的SCFA通过逻辑回归进行鉴定，并通过随机森林分析进行排序。
结果：将86个植入物分为种植体周围健康组（PIH组，35个植入物），种植体周围黏膜炎组(PIM组，25个植入物），和种植体周围炎组(PI组，26个植入物）根据临床和影像学检查结果。PIM组的甲酸检出率明显低于其他两组（p<0.001）。PIM组的乙酸水平明显较高，丙酸,和异戊酸高于PIH组(p<0.05)。PI组的丙酸水平明显较高，丁酸，异丁酸,valeric,和异戊酸高于PIH组(p<0.05)。PI组的丁酸水平明显较高，异丁酸,和异戊酸比PIM组（p<0.05）。SCFA(除己酸和琥珀酸外)与临床参数显著正相关(p<0.05)。与种植体周围疾病相关的SCFA排名如下：丁酸，异戊，异丁酸,丙酸,乙酸,甲酸,和乳酸。
结论：特异性SCFA升高与种植体周围疾病相关。识别这种相关性可能有助于早期识别植入物周围疾病并指导进一步的临床干预。
方法：从已放置至少5年的植入物中获得PISF样品，和种植体周围的临床参数进行了检查。采用气相色谱-质谱法和高效液相色谱法检测PISF中的SCFA。采用Spearman相关性分析SCFAs与临床参数的相关性。与种植体周围疾病相关的SCFA通过逻辑回归进行鉴定，并通过随机森林分析进行排序。
结果：将86个植入物分为种植体周围健康组（PIH组，35个植入物），种植体周围黏膜炎组(PIM组，25个植入物），和种植体周围炎组(PI组，26个植入物）根据临床和影像学检查结果。PIM组的甲酸检出率明显低于其他两组（p<0.001）。PIM组的乙酸水平明显较高，丙酸,和异戊酸高于PIH组(p<0.05)。PI组的丙酸水平明显较高，丁酸，异丁酸,valeric,和异戊酸高于PIH组(p<0.05)。PI组的丁酸水平明显较高，异丁酸,和异戊酸比PIM组（p<0.05）。SCFA(除己酸和琥珀酸外)与临床参数显著正相关(p<0.05)。与种植体周围疾病相关的SCFA排名如下：丁酸，异戊，异丁酸,丙酸,乙酸,甲酸,和乳酸。
结论：特异性SCFA升高与种植体周围疾病相关。识别这种相关性可能有助于早期识别植入物周围疾病并指导进一步的临床干预。
