Abstract：
Photoaging, caused by ultraviolet (UV) radiation, is characterized by the senescence of skin cells and reduction of collagens. Although rice fermentation is widely used in the cosmetics, its impact on skin photoaging is still not well understood. Herein, we investigated the possible effectiveness of Maifuyin, a fermented rice product, and its components, succinic acid (SA), and choline, for safeguarding UVA-exposed human dermal fibroblasts (HDFs) against photoaging. In this study, the effects of Maifuyin, SA, and choline on UVA-induced cell death and senescence in fibroblasts were evaluated in cell counting kit-8 (CCK-8), expression of β-galactosidase (β-GAL), and matrix metalloproteinases (MMP)-1. To identify oxidative stress, the investigation focused on reactive oxygen species, glutathione, superoxide dismutase, and malondialdehyde. Additionally, a mRNA sequencing technology (RNA-seq) was applied to study the underlying mechanisms of these components on UVA-induced photoaging. Meanwhile, the level of C-X-C motif chemokine ligand 2 (CXCL2) in the cell supernatant was confirmed to assess the autocrine chemokine level. To reassess the involvement of CXCL2, the expression of β-GAL was evaluated in fibroblasts treated with or without CXCL2. The results indicated that 1 mg/mL Maifuyin and SA inhibited UVA-induced senescence in fibroblasts, MMP-1 expression, and oxidative damage. The RNA-seq revealed 1 mg/mL Maifuyin and SA might be recruited chemokine CXCLs to inhibit MMPs production and fibroblast senescence via TNFα, MAPK, and NF-κB pathways. ELISA results showed a significant reduction of autocrine CXCL2 in UVA-irradiated HDFs by pretreating Maifuyin and SA. The β-GAL staining assay revealed that CXCL2 treatment increased β-GAL activity, while the administration of Maifuyin and SA counteracted this effect in HDFs. These results highlighted the potential use of Maifuyin and SA as promising candidates for anti-photoaging applications.
摘要：
光老化,由紫外线（UV）辐射引起，其特征是皮肤细胞衰老和胶原蛋白减少。尽管大米发酵在化妆品中被广泛使用，它对皮肤光老化的影响仍然没有很好的理解。在这里，我们调查了麦福音的可能有效性，发酵大米产品，及其组成部分，琥珀酸(SA),还有胆碱,用于保护暴露于UVA的人真皮成纤维细胞（HDFs）免受光老化。在这项研究中,麦福音的影响，SA,在细胞计数试剂盒-8（CCK-8）中评估了胆碱对UVA诱导的成纤维细胞中细胞死亡和衰老的影响，β-半乳糖苷酶（β-GAL）的表达，和基质金属蛋白酶(MMP)-1。为了识别氧化应激，调查的重点是活性氧，谷胱甘肽,超氧化物歧化酶,还有丙二醛.此外,应用mRNA测序技术(RNA-seq)研究这些组分对UVA诱导的光老化的潜在机制.同时,确认细胞上清液中C-X-C基序趋化因子配体2(CXCL2)的水平以评估自分泌趋化因子水平.为了重新评估CXCL2的参与,在用或不用CXCL2处理的成纤维细胞中评估β-GAL的表达。结果表明，1mg/mL麦复饮和SA抑制UVA诱导的成纤维细胞衰老，MMP-1表达,和氧化损伤。RNA-seq显示1mg/mL的麦复阴和SA可能通过TNFα募集趋化因子CXCL来抑制MMPs的产生和成纤维细胞衰老，MAPK,和NF-κB通路。ELISA结果显示，通过预处理Maifuyin和SA，UVA照射的HDFs中自分泌CXCL2的显着减少。β-GAL染色分析显示CXCL2处理增加β-GAL活性,而在HDF中，麦复音和SA的给药抵消了这种作用。这些结果强调了Maifuyin和SA作为抗光老化应用的有希望的候选物的潜在用途。
