PDF(Pubmed)
Abstract：
Neokestose is considered to have a prebiotic function. However, the physiological activity of neokestose remains unknown. Neokestose has a blastose, a sucrose analog, in its structure. We previously demonstrated that oral administration of blastose to diabetic rats suppressed the increase in plasma glucose (PG) concentration after sucrose administration. Therefore, neokestose might have a similar effect. In this study, we investigated the effects of neokestose on PG concentrations and the mechanism of its action. We first administered neokestose orally to streptozotocin-induced diabetic rats and observed that the expected consequent increase in PG concentration was significantly suppressed. Next, we examined the inhibitory effect of neokestose on glycosidase activity, but observed only a slight inhibitory effect. Therefore, we hypothesized that neokestose might be hydrolyzed by gastric acid to produce blastose. We performed an acid hydrolysis of neokestose using artificial gastric juice. After acid hydrolysis, peaks corresponding to neokestose and its decomposition products including blastose were observed. Therefore, we suggest that neokestose and blastose, a decomposition product, synergistically inhibit glycosidase activity. These findings support the potential use of neokestose as a useful functional oligosaccharide that can help manage plasma glucose concentrations in patients with diabetes mellitus.
摘要：
Neokestose被认为具有益生元功能。然而,中性粒细胞的生理活性仍然未知。Neokestose有一个囊胚，蔗糖类似物，在其结构中。我们先前证明，向糖尿病大鼠口服囊胚糖可以抑制蔗糖给药后血浆葡萄糖（PG）浓度的增加。因此,neokestose可能有类似的效果。在这项研究中,我们研究了中性粒细胞对PG浓度的影响及其作用机理。我们首先对链脲佐菌素诱导的糖尿病大鼠口服注射中性粒细胞，并观察到预期的PG浓度随之增加被显着抑制。接下来,我们研究了Nokestose对糖苷酶活性的抑制作用，但只观察到轻微的抑制作用。因此,我们假设中性雌酚可能被胃酸水解产生胚泡糖。我们使用人工胃液对新氧雌酚进行了酸水解。酸水解后，观察到对应于中性雌酚及其分解产物(包括胚泡糖)的峰。因此,我们建议nokestose和blastose，分解产物，协同抑制糖苷酶活性。这些发现支持了作为一种有用的功能性寡糖的新雌雄糖的潜在用途，可以帮助控制糖尿病患者的血浆葡萄糖浓度。
