Abstract：
Low-coverage whole genome sequencing was performed for tissue samples from thyroid patients who received surgery treatment from 2015 to 2021. The potential biological significance of CD147 protein in thyroid cancer was explored through correlation analysis of CD147 protein expression level and clinical features of thyroid cancer patients. Low coverage whole genome sequencing was performed on the extracted DNA samples. The copy number analysis software was used to analyze the sequencing data, calculate the copy number of CD147 gene, further verify the expression of CD147 gene, and analyze its association with clinical features. The relationship between CIN and high risk was evaluated in the internal cohort. The association of CIN with the disease-free survival was validated in the cohort from The Cancer Genome Atlas Program. Thyroglobulin plays a key role in regulating thyroid function and maintaining normal metabolic rate. By sequencing tissue samples from this study, we can gain a deeper understanding of the association between cin and thyroid disease. The percentage of high risk patients in the multiple CIN group (77.8 %) was significantly higher than that in the 22q negative group (31.3 %), BRAF V600E group (22.2 %) and all negative group (25.0 %; p = 0.043).
摘要：
对2015年至2021年接受手术治疗的甲状腺患者的组织样本进行了低覆盖率全基因组测序。通过对甲状腺癌患者CD147蛋白表达水平与临床特征的相关性分析,探讨CD147蛋白在甲状腺癌中的潜在生物学意义。对提取的DNA样品进行低覆盖全基因组测序。使用拷贝数分析软件对测序数据进行分析,计算CD147基因的拷贝数，进一步验证CD147基因的表达,并分析其与临床特征的关系。在内部队列中评估CIN与高风险之间的关系。CIN与无病生存率的关联在癌症基因组图谱计划的队列中得到了验证。甲状腺球蛋白在调节甲状腺功能和维持正常代谢率中起关键作用。通过对这项研究的组织样本进行测序，我们可以更深入地了解cin与甲状腺疾病之间的关系。MultipleCIN组的高危患者比例(77.8%)明显高于22q阴性组(31.3%),BRAFV600E组(22.2%)和全体阴性组(25.0%;p=0.043)。
