Abstract：
Three novel crayfish-infecting nudiviruses from crayfish in North America represent the first genomic confirmation of nudiviruses in crayfish: Faxonius propinquus nudivirus (FpNV), Faxonius rusticus nudivirus (FrNV), and Faxonius virilis nudivirus (FvNV). Histopathology and electron microscopy revealed nuclear infections, including nuclear hypertrophy in hepatopancreatic epithelial cells and the presence of membrane-bound bacilliform virions. Metagenomic sequencing resulted in complete circular genome assembly, and phylogenetic analyses (based on nudivirus core genes) placed these viruses within the unofficial Epsilonnudivirus genus. One of the nudiviruses was detected in the antennal gland of its host, and another is correlated with invasive crayfish decline in one infected lake ecosystem - suggesting a potential route for viral transmission through water, and possible population level impact. This study highlights the importance of genomic and ecological data in elucidating the diversity and evolutionary relationships of the Nudiviridae, while expanding their known diversity and range of host species.
摘要：
来自北美小龙虾的三种新型小龙虾感染的裸病毒代表了小龙虾中裸病毒的首次基因组确认：Faxoniusproprecinkusnumdivirus（FpNV），法克索尼乌斯病毒（FrNV），和法克森尼乌斯病毒（FvNV）。组织病理学和电子显微镜显示核感染，包括肝胰腺上皮细胞的核肥大和膜结合的杆状病毒体的存在。宏基因组测序导致完整的环状基因组组装，和系统发育分析（基于裸病毒核心基因）将这些病毒置于非官方的Epsilonnodivirus属中。其中一种病毒在其宿主的触角腺中被检测到，另一个与一个受感染的湖泊生态系统中侵入性小龙虾的下降有关-这表明病毒通过水传播的潜在途径，以及可能的人口水平影响。这项研究强调了基因组和生态数据在阐明Nutriridae的多样性和进化关系中的重要性，同时扩大其已知的宿主物种多样性和范围。
