Abstract：
Atomic force microscopy is a high-resolution imaging technique useful for observing the structures of biomolecular complexes. This approach provides a straightforward method to characterize the binding behavior of different chromatin architectural proteins and to analyze the increasingly complex structural units assembled on the DNA. The protocol describes the preparation, AFM imaging, and structural analysis of chromatin that is reconstituted in vitro using purified proteins and DNA. Here, we describe the successful application of the method on the chromatin architectural proteins of the archaeon Sulfolobus solfataricus.
摘要：
原子力显微镜是一种高分辨率成像技术，可用于观察生物分子复合物的结构。这种方法提供了一种直接的方法来表征不同染色质结构蛋白的结合行为，并分析DNA上组装的日益复杂的结构单元。协议描述了准备工作，AFM成像,以及使用纯化的蛋白质和DNA在体外重建的染色质的结构分析。这里,我们描述了该方法在古细菌Sulfolobussolfataricus的染色质结构蛋白上的成功应用。
