Abstract：
The development of novel DNA assembly methods in recent years has paved the way for the construction of synthetic replicons to be used for basic research and biotechnological applications. A learning-by-building approach can now answer questions about how chromosomes must be constructed to maintain genetic information. Here we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in Escherichia coli based on the popular modular cloning (MoClo) system.
摘要：
近年来，新型DNA组装方法的发展为构建用于基础研究和生物技术应用的合成复制子铺平了道路。构建学习方法现在可以回答有关如何构建染色体以维持遗传信息的问题。在这里，我们描述了一个有效的管道，用于设计和组装合成，基于流行的模块化克隆（MoClo）系统的大肠杆菌次级染色体。
