Abstract：
Seven bacterial strains, isolated from various Tunisian biotopes, were investigated for Congo Red (CR) and Malachite Green (MG) decolorization. The isolated strains underwent morphological and biochemical tests, including assessments for antibiotic sensitivity as well as biofilm formation. One selected strain, ST11, was partially identified as Paenibacillus sp. strain ST11. The newly isolated crude bacterial filtrates (NICBFs) effectively decolorized CR and MG. Specifically, six and seven NICBFs were found to be effective for degrading CR (150 mg l-1) and MG (50 mg l-1), respectively. Under non-optimized conditions, CR and MG could be decolorized up to 80% within 6-12 h. The degradation products of CR and MG, characterized by UV-visible and FT-IR techniques, demonstrated both decolorization and transformation, highlighting the role of enzymes in dye degradation. Phytotoxicity and cytotoxicity studies evaluated the impact of treated and untreated CR and MG. Some NICBFs showed promise as powerful biological tools, reducing and sometimes detoxifying CR and MG, commonly used as fertilizers. The potential applications of these NICBFs in decolorization and bioremediation of dye-rich textile effluents were explored. The screening also identified environmentally friendly, cost-effective bacterial strains adaptable to various conditions through phytotoxicity and cytotoxicity studies.
摘要：
七种细菌菌株，从各种突尼斯生物群落中分离出来，研究了刚果红(CR)和孔雀石绿(MG)的脱色效果。对分离出的菌株进行了形态学和生化检测,包括评估抗生素的敏感性以及生物膜的形成。一个选定的菌株，ST11被部分鉴定为类芽孢杆菌。菌株ST11.新分离的粗细菌滤液（NICBFs）有效地脱色了CR和MG。具体来说,发现六个和七个NICBF对降解CR（150mgl-1）和MG（50mgl-1）有效，分别。在非优化条件下，CR和MG可在6-12h内脱色80%。通过紫外可见和FT-IR技术表征，表现出脱色和转化，强调酶在染料降解中的作用。植物毒性和细胞毒性研究评估了处理的和未处理的CR和MG的影响。一些NICBF显示出作为强大生物工具的前景，减少并有时排毒CR和MG，通常用作肥料。探索了这些NICBF在富含染料的纺织品废水的脱色和生物修复中的潜在应用。筛查还确定了环保，通过植物毒性和细胞毒性研究，具有成本效益的细菌菌株可适应各种条件。
