Abstract：
Intravascularly transplanted bone marrow cells, including bone marrow mononuclear cells (BM-MNC) and mesenchymal stem cells, transfer water-soluble molecules to cerebral endothelial cells via gap junctions. After transplantation of BM-MNC, this fosters hippocampal neurogenesis and enhancement of neuronal function. Herein, we report the impact of transplanted BM-MNC on neural stem cells (NSC) in the brain. Surprisingly, direct transfer of water-soluble molecules from transplanted BM-MNC and peripheral mononuclear cells to NSC in the hippocampus was observed already 10 min after cell transplantation, and transfer from BM-MNC to GFAP-positive cortical astrocytes was also observed. In vitro investigations revealed that BM-MNC abolish the expression of hypoxia-inducible factor-1α in astrocytes. We suggest that the transient and direct transfer of water-soluble molecules between cells in circulation and NSC in the brain may be one of the biological mechanisms underlying the repair of brain function.
摘要：
血管内移植的骨髓细胞，包括骨髓单个核细胞（BM-MNC）和间充质干细胞，通过缝隙连接将水溶性分子转移到脑内皮细胞。移植BM-MNC后，这促进了海马神经发生和神经元功能的增强。在这里，我们报道了移植的BM-MNC对脑中神经干细胞（NSC）的影响。令人惊讶的是,细胞移植后10分钟，观察到水溶性分子从移植的BM-MNC和外周单核细胞直接转移到海马中的NSC，并且还观察到从BM-MNC向GFAP阳性皮质星形胶质细胞的转移。体外研究表明，BM-MNC消除了星形胶质细胞中HIF1α的表达。我们认为，循环中的细胞和大脑中的NSC之间的水溶性分子的瞬时和直接转移可能是脑功能修复的生物学机制之一。
