PDF(Pubmed)
Abstract：
UNASSIGNED: Hepatocellular carcinoma (HCC) is one of the most serious types of cancer that accounts for numerous cancer deaths worldwide. HCC is poorly prognosed and is a highly chemotherapy-resistant tumor. Therefore, new treatments are urgently needed. Exopolysaccharides (EPS-1) produced from the novel Bacillus sonorensis strain was found to exhibit chemopreventive effects against cancer.
UNASSIGNED: Evaluating the anti-cancer cytotoxic effect of exopolysaccharides (EPS-1) produced by the newly studied Bacillus sonorensis strain SAmt2.
UNASSIGNED: The cytotoxic activity was investigated through cell cycle, apoptosis, and autophagy analyses using flow cytometry technique. Also, the effect of EPS-1 on Huh7 release of COX-2 was examined using ELISA.
UNASSIGNED: Our results revealed that EPS-1exhibit an anti-proliferative effect on Huh7 cells through decreasing the percentage of cells at the S-phase and G2 phase, while increasing the cell population at the sub-G1 and G1 phases. Apoptosis analysis showed that EPS-1 increased necrotic and apoptotic cell fractions in EPS-1 treated Huh7. In addition, it induced significant autophagic cell death in the Huh7.Finally, antiproliferative and apoptosis induction results were supportedby ELISA assay results where the protein level of COX-2 was declined.
UNASSIGNED: : In conclusion, EPS-1 derived from B. sonorensis SAmt2, is a promising proliferation inhibitor of Huh7 cells with potential anticancer effects.
UNASSIGNED: Evaluating the anti-cancer cytotoxic effect of exopolysaccharides (EPS-1) produced by the newly studied Bacillus sonorensis strain SAmt2.
UNASSIGNED: The cytotoxic activity was investigated through cell cycle, apoptosis, and autophagy analyses using flow cytometry technique. Also, the effect of EPS-1 on Huh7 release of COX-2 was examined using ELISA.
UNASSIGNED: Our results revealed that EPS-1exhibit an anti-proliferative effect on Huh7 cells through decreasing the percentage of cells at the S-phase and G2 phase, while increasing the cell population at the sub-G1 and G1 phases. Apoptosis analysis showed that EPS-1 increased necrotic and apoptotic cell fractions in EPS-1 treated Huh7. In addition, it induced significant autophagic cell death in the Huh7.Finally, antiproliferative and apoptosis induction results were supportedby ELISA assay results where the protein level of COX-2 was declined.
UNASSIGNED: : In conclusion, EPS-1 derived from B. sonorensis SAmt2, is a promising proliferation inhibitor of Huh7 cells with potential anticancer effects.
摘要：
■肝细胞癌（HCC）是最严重的癌症之一，在全球范围内导致许多癌症死亡。HCC预后不良，是一种高度耐药的肿瘤。因此,迫切需要新的治疗方法。发现由新型超声芽孢杆菌菌株产生的胞外多糖（EPS-1）表现出针对癌症的化学预防作用。
■评估新研究的超声波芽孢杆菌菌株SAmt2产生的胞外多糖（EPS-1）的抗癌细胞毒性作用。
■通过细胞周期研究细胞毒性活性，凋亡，和自噬分析使用流式细胞术技术。此外，采用ELISA法检测EPS-1对Huh7释放COX-2的影响。
■我们的结果表明，EPS-1通过降低S期和G2期细胞的百分比，对Huh7细胞具有抗增殖作用，同时增加亚G1期和G1期的细胞群。凋亡分析显示EPS-1增加EPS-1处理的Huh7中的坏死和凋亡细胞分数。此外,它在Huh7中诱导了显著的自噬性细胞死亡。最后,抗增殖和凋亡诱导结果由ELISA测定结果支持，其中COX-2蛋白水平下降。
■：总之，来自B.sonorensisSAmt2的EPS-1是一种有前途的Huh7细胞增殖抑制剂，具有潜在的抗癌作用。
■评估新研究的超声波芽孢杆菌菌株SAmt2产生的胞外多糖（EPS-1）的抗癌细胞毒性作用。
■通过细胞周期研究细胞毒性活性，凋亡，和自噬分析使用流式细胞术技术。此外，采用ELISA法检测EPS-1对Huh7释放COX-2的影响。
■我们的结果表明，EPS-1通过降低S期和G2期细胞的百分比，对Huh7细胞具有抗增殖作用，同时增加亚G1期和G1期的细胞群。凋亡分析显示EPS-1增加EPS-1处理的Huh7中的坏死和凋亡细胞分数。此外,它在Huh7中诱导了显著的自噬性细胞死亡。最后,抗增殖和凋亡诱导结果由ELISA测定结果支持，其中COX-2蛋白水平下降。
■：总之，来自B.sonorensisSAmt2的EPS-1是一种有前途的Huh7细胞增殖抑制剂，具有潜在的抗癌作用。
