PDF(Pubmed)
Abstract：
UNASSIGNED: Dengue is one of the important vector-borne viral diseases affecting humans with diverse manifestations. Toll-like receptors (TLR) are pattern recognition receptors and play an important role in innate immunity against microbes. TLR3 plays a critical role in controlling the innate immune response mediated by flaviviruses such as dengue.
UNASSIGNED: We attempted to study the susceptibility of single nucleotide polymorphism of the TLR3 gene in dengue encephalitis (DE) patients and determine the association in terms of genotype, allele, and haplotype distribution along with the clinical outcome.
UNASSIGNED: It was a case-controlled observational study in a tertiary care hospital.
UNASSIGNED: We investigated the single nucleotide polymorphism in the TLR3 Leu412Phe gene using real-time polymerase chain reaction in 29 cases of DE and compared them with equal number of age- and sex-matched dengue patients without neurological features.
UNASSIGNED: The genotype and allele frequencies were compared using a two-sided Chi-square or Fisher\'s exact test.
UNASSIGNED: The findings revealed that the genotypic distribution of TLR3 Leu412Phe polymorphism for the mutant genotype Phe/Phe (TT) demonstrated increased association of DE (31.03% vs 6.8%, P 0.019, odds ratio 6.075, 95% confidence interval 1.181-31.245). However, the number of heterozygous (H) genotype (Leu/Phe-CT) and mutant Phe allele (T) did not show any statistically significant association. TLR3 gene polymorphism did not show any correlation with mortality outcome at 1 month.
UNASSIGNED: The presence of mutant TLR3 Leu412Phe polymorphism may confer the propensity to have DE in patients with dengue infection in the Indian population. TLR3 polymorphism did not affect mortality outcome at 1 month.
UNASSIGNED: We attempted to study the susceptibility of single nucleotide polymorphism of the TLR3 gene in dengue encephalitis (DE) patients and determine the association in terms of genotype, allele, and haplotype distribution along with the clinical outcome.
UNASSIGNED: It was a case-controlled observational study in a tertiary care hospital.
UNASSIGNED: We investigated the single nucleotide polymorphism in the TLR3 Leu412Phe gene using real-time polymerase chain reaction in 29 cases of DE and compared them with equal number of age- and sex-matched dengue patients without neurological features.
UNASSIGNED: The genotype and allele frequencies were compared using a two-sided Chi-square or Fisher\'s exact test.
UNASSIGNED: The findings revealed that the genotypic distribution of TLR3 Leu412Phe polymorphism for the mutant genotype Phe/Phe (TT) demonstrated increased association of DE (31.03% vs 6.8%, P 0.019, odds ratio 6.075, 95% confidence interval 1.181-31.245). However, the number of heterozygous (H) genotype (Leu/Phe-CT) and mutant Phe allele (T) did not show any statistically significant association. TLR3 gene polymorphism did not show any correlation with mortality outcome at 1 month.
UNASSIGNED: The presence of mutant TLR3 Leu412Phe polymorphism may confer the propensity to have DE in patients with dengue infection in the Indian population. TLR3 polymorphism did not affect mortality outcome at 1 month.
摘要：
■登革热是影响人类的重要媒介传播的病毒性疾病之一，表现多样。Toll样受体（TLR）是模式识别受体，在抵抗微生物的先天免疫中起重要作用。TLR3在控制由黄病毒如登革热介导的先天免疫应答中起关键作用。
■我们试图研究登革热脑炎（DE）患者TLR3基因单核苷酸多态性的易感性，并确定基因型的相关性，等位基因,和单倍型分布以及临床结果。
■这是一项三级医院的病例对照观察性研究。
■我们使用实时聚合酶链反应研究了29例DE患者的TLR3Leu412Phe基因的单核苷酸多态性，并将其与相同数量的年龄和性别匹配的登革热患者进行了比较没有神经系统特征。
■使用双侧卡方或Fisher精确检验比较基因型和等位基因频率。
■研究结果表明，突变基因型Phe/Phe（TT）的TLR3Leu412Phe多态性的基因型分布表明DE的相关性增加（31.03％vs6.8％，P0.019,比值比6.075,95%置信区间1.181-31.245)。然而,杂合(H)基因型(Leu/Phe-CT)和突变型Phe等位基因(T)的数量未显示任何统计学显著关联.TLR3基因多态性与1个月时的死亡率无相关性。
■突变TLR3Leu412Phe多态性的存在可能赋予印度人群登革热感染患者患有DE的倾向。TLR3多态性不影响1个月时的死亡率结果。
■我们试图研究登革热脑炎（DE）患者TLR3基因单核苷酸多态性的易感性，并确定基因型的相关性，等位基因,和单倍型分布以及临床结果。
■这是一项三级医院的病例对照观察性研究。
■我们使用实时聚合酶链反应研究了29例DE患者的TLR3Leu412Phe基因的单核苷酸多态性，并将其与相同数量的年龄和性别匹配的登革热患者进行了比较没有神经系统特征。
■使用双侧卡方或Fisher精确检验比较基因型和等位基因频率。
■研究结果表明，突变基因型Phe/Phe（TT）的TLR3Leu412Phe多态性的基因型分布表明DE的相关性增加（31.03％vs6.8％，P0.019,比值比6.075,95%置信区间1.181-31.245)。然而,杂合(H)基因型(Leu/Phe-CT)和突变型Phe等位基因(T)的数量未显示任何统计学显著关联.TLR3基因多态性与1个月时的死亡率无相关性。
■突变TLR3Leu412Phe多态性的存在可能赋予印度人群登革热感染患者患有DE的倾向。TLR3多态性不影响1个月时的死亡率结果。
