PDF(Pubmed)
Abstract：
Micro ribonucleic acids (miRNAs) are a highly conserved class of single-stranded non-coding RNAs. Within the miR-545/374a cluster, miR-545 resides in the intron of the long non-coding RNA (lncRNA) FTX on Xq13.2. The precursor form, pre-miR-545, is cleaved to generate two mature miRNAs, miR-545-3p and miR-545-5p. Remarkably, these two miRNAs exhibit distinct aberrant expression patterns in different cancers; however, their expression in colorectal cancer remains controversial. Notably, miR-545-3p is affected by 15 circular RNAs (circRNAs) and 10 long non-coding RNAs (lncRNAs), and it targets 27 protein-coding genes (PCGs) that participate in the regulation of four signaling pathways. In contrast, miR-545-5p is regulated by one circRNA and five lncRNAs, it targets six PCGs and contributes to the regulation of one signaling pathway. Both miR-545-3p and miR-545-5p affect crucial cellular behaviors, including cell cycle, proliferation, apoptosis, epithelial-mesenchymal transition, invasion, and migration. Although low miR-545-3p expression is associated with poor prognosis in three cancer types, studies on miR-545-5p are yet to be reported. miR-545-3p operates within a diverse range of regulatory networks, thereby augmenting the efficacy of cancer chemotherapy, radiotherapy, and immunotherapy. Conversely, miR-545-5p enhances immunotherapy efficacy by inhibiting T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) expression. In summary, miR-545 holds immense potential as a cancer biomarker and therapeutic target. The aberrant expression and regulatory mechanisms of miR-545 in cancer warrant further investigation.
摘要：
微核糖核酸(miRNA)是一类高度保守的单链非编码RNA。在miR-545/374a簇内，miR-545位于Xq13.2上的长链非编码RNA（lncRNA）FTX的内含子中。前体形式，pre-miR-545被切割以产生两个成熟的miRNA，miR-545-3p和miR-545-5p。值得注意的是,这两种miRNA在不同的癌症中表现出不同的异常表达模式;然而,它们在结直肠癌中的表达仍存在争议.值得注意的是,miR-545-3p受15个环状RNA（circRNAs）和10个长链非编码RNA（lncRNAs）的影响，它靶向27个蛋白质编码基因（PCGs），参与4个信号通路的调节。相比之下,miR-545-5p受一个circRNA和五个lncRNAs调控，它靶向六个PCGs，并有助于一个信号通路的调节。miR-545-3p和miR-545-5p都影响关键的细胞行为，包括细胞周期，扩散,凋亡，上皮-间质转化，入侵,和移民。尽管低miR-545-3p表达与三种癌症类型的不良预后相关，关于miR-545-5p的研究尚未报道。miR-545-3p在多种调控网络中运作，从而增强癌症化疗的疗效，放射治疗,和免疫疗法。相反，miR-545-5p通过抑制T细胞免疫球蛋白和含粘蛋白结构域3(TIM-3)表达来增强免疫疗法功效。总之,miR-545作为癌症生物标志物和治疗靶标具有巨大潜力。miR-545在癌症中的异常表达和调控机制需要进一步研究。
