Abstract：
This study presents a comprehensive genomic exploration, biochemical characterization, and the identification of antibiotic resistance and specialty genes of Pediococcus acidilactici BCB1H strain. The functional characterization, genetic makeup, biological activities, and other considerable parameters have been investigated in this study with a prime focus on antibiotic resistance and specialty gene profiles. The results of this study revealed the unique susceptibility patterns for antibiotic resistance and specialty genes. BCB1H had good in vitro probiotic properties, which survived well in simulated artificial gastrointestinal fluid, and exhibited acid and bile salt resistance. BCB1H didn\'t produce hemolysis and had certain antibiotic sensitivity, making it a relatively safe LAB strain. Simultaneously, it had good self-coagulation characteristics and antioxidant activity. The EPS produced by BCB1H also had certain antioxidant activity and hypoglycemic function. Moreover, the genome with a 42.4 % GC content and a size of roughly 1.92 million base pairs was analyzed in the genomic investigations. The genome annotation identified 192 subsystems and 1,895 genes, offering light on the metabolic pathways and functional categories found in BCB1H. The identification of specialty genes linked to the metabolism of carbohydrates, stress response, pathogenicity, and amino acids highlighted the strain\'s versatility and possible uses. This study establishes the groundwork for future investigations by highlighting the significance of using multiple strains to investigate genetic diversity and experimental validation of predicted genes. The results provide a roadmap for utilizing P. acidilactici BCB1H\'s genetic traits for industrial and medical applications, opening the door to real-world uses in industries including food technology and medicine.
摘要：
这项研究提出了一个全面的基因组探索，生化表征，酸乳片球菌BCB1H菌株的耐药性和特异性基因的鉴定。功能表征，基因构成,生物活动，在这项研究中，已经研究了其他相当多的参数，主要关注抗生素耐药性和特殊基因谱。这项研究的结果揭示了抗生素抗性和特殊基因的独特易感性模式。BCB1H具有良好的体外益生特性,在模拟的人工胃肠液中存活良好，并表现出耐酸和耐胆盐。BCB1H不产生溶血，有一定的抗生素敏感性，使其成为相对安全的LAB菌株。同时,具有良好的自凝特性和抗氧化活性。BCB1H产生的EPS也具有一定的抗氧化活性和降血糖功能。此外，在基因组研究中分析了GC含量为42.4％，大小约为192万个碱基对的基因组。基因组注释确定了192个子系统和1,895个基因，提供BCB1H中发现的代谢途径和功能类别的光。鉴定与碳水化合物代谢相关的特殊基因，应激反应，致病性，和氨基酸强调了菌株的多功能性和可能的用途。本研究通过强调使用多个菌株研究遗传多样性和预测基因的实验验证的重要性，为未来的研究奠定了基础。研究结果为利用酸乳杆菌BCB1H的遗传性状用于工业和医学应用提供了路线图，为食品技术和医药等行业的实际应用打开了大门。
