Abstract：
Pamphagidae is a family of Acridoidea that inhabits the desert steppes of Eurasia and Africa. This study employed flow cytometry to estimate the genome size of eight species in the Pamphagidae. The results indicate that the genome size of the eight species ranged from 13.88 pg to 14.66 pg, with an average of 14.26 pg. This is the largest average genome size recorded for the Orthoptera families, as well as for the entire Insecta. Furthermore, the study explored the role of repetitive sequences in the genome, including their evolutionary dynamics and activity, using low-coverage next-generation sequencing data. The genome is composed of 14 different types of repetitive sequences, which collectively make up between 59.9% and 68.17% of the total genome. The Pamphagidae family displays high levels of transposable element (TE) activity, with the number of TEs increasing and accumulating since the family\'s emergence. The study found that the types of repetitive sequences contributing to the TE outburst events are similar across species. Additionally, the study identified unique repetitive elements for each species. The differences in repetitive sequences among the eight Pamphagidae species correspond to their phylogenetic relationships. The study sheds new light on genome gigantism in the Pamphagidae and provides insight into the correlation between genome size and repetitive sequences within the family.
摘要：
Pamphagidae是Acridoidea家族，栖息在欧亚大陆和非洲的沙漠草原上。这项研究采用流式细胞术估算了Pamphagidae中八个物种的基因组大小。结果表明，这8个物种的基因组大小在13.88pg至14.66pg之间，平均14.26pg。这是直翅目家族记录的最大平均基因组大小，以及整个昆虫。此外，这项研究探索了重复序列在基因组中的作用，包括它们的进化动力学和活动，使用低覆盖率的下一代测序数据。基因组由14种不同类型的重复序列组成，它们总共占总基因组的59.9%至68.17%。Pamphagidae家族表现出高水平的转座因子（TE）活性，随着家庭出现以来TEs数量的增加和积累。研究发现，导致TE爆发事件的重复序列类型在物种之间相似。此外,这项研究为每个物种确定了独特的重复元素。八个Pamphagidae物种之间重复序列的差异与其系统发育关系相对应。该研究为Pamphagidae的基因组巨人症提供了新的思路，并提供了对基因组大小与家族内重复序列之间相关性的见解。
