Abstract：
Generating stable and customizable topography on hydrogel surfaces with contact guidance potential is critical as it can direct/influence cell growth. This necessitates the development of new techniques for surface patterning of the hydrogels. We report on the design of a square grid template for surface patterning hydrogels. The template was 3-D printed and has the diameter of a well in a 24-well plate. Hyaluronic acid methacrylate (HA) hydrogel precursor solutions were cast on the 3D printed template\'s surface, which generated 3D square shape topographies on the HA hydrogel surface upon demolding. The 3D Laser Microscopy has shown the formation of a periodic array of 3D topographies on hydrogel surfaces. 3D Laser and Electron Microscopy Imaging have revealed that this new method has increased the surface area and exposed the underlying pore structure of the HA hydrogels. To demonstrate the method\'s versatility, we have successfully applied this technique to generate 3D topography on two more acrylate hydrogel formulations, gelatin Methacrylate and polyethylene glycol dimethacrylate. Human neonatal dermal fibroblast cells were used as a model cell line to evaluate the cell guidance potential of patterned HA hydrogel. Confocal fluorescence microscopy imaging has revealed that the 3D surface topographies on HA hydrogels can guide and align the actin filaments of the fibroblasts presumably due to the contact guidance mechanism. The newly developed methodology of 3D topography generation in acrylate hydrogels may influence the cell responses on hydrogel surfaces which can impact biomedical applications such as tissue engineering, wound healing, and disease modeling.
摘要：
在具有接触引导电位的水凝胶表面上产生稳定且可定制的形貌是关键的,因为它可以引导/影响细胞生长。这需要开发用于水凝胶的表面图案化的新技术。我们报告了用于表面图案化水凝胶的正方形网格模板的设计。模板是3D打印的并且具有24孔板中的孔的直径。透明质酸甲基丙烯酸酯（HA）水凝胶前体溶液浇铸在3D打印模板的表面，其在脱模时在HA水凝胶表面上产生3D正方形形貌。3D激光显微镜已显示在水凝胶表面上形成3D形貌的周期性阵列。3D激光和电子显微镜成像显示，这种新方法增加了HA水凝胶的表面积并暴露了其潜在的孔结构。为了证明该方法的多功能性，我们已经成功地将这种技术应用于在两种以上的丙烯酸酯水凝胶配方上生成3D形貌，明胶甲基丙烯酸酯和聚乙二醇二甲基丙烯酸酯。使用人新生儿真皮成纤维细胞作为模型细胞系来评估图案化HA水凝胶的细胞引导潜力。共聚焦荧光显微镜成像显示，HA水凝胶上的3D表面形貌可以引导和对齐成纤维细胞的肌动蛋白丝，这可能是由于接触引导机制。新开发的丙烯酸酯水凝胶3D形貌生成方法可能会影响水凝胶表面的细胞反应，从而影响生物医学应用，如组织工程。伤口愈合，和疾病建模。
