UNASSIGNED: A set of 284 mango accessions from different regions of the world were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction to generate genomic single-nucleotide polymorphism (SNP).
UNASSIGNED: After filtering, raw data containing 539.61 M reads were obtained. A total of 505,300 SLAFs were detected, of which, 205,299 were polymorphic. Finally, 29,136 SNPs were employed to dissect the population structure, genetic relationships, and genetic diversity. The 284 mango accessions were divided into two major groups: one group consisted mainly of mango accessions from Australia, the United States, Cuba, India, Caribbean, Israel, Pakistan, Guinea, Burma, China, and Sri Lanka, which belonged to the Indian type (P1); the other group contained mango accessions from the Philippines, Thailand, Indonesia, Vietnam, Cambodia, Malaysia, and Singapore, which belonged to Southeast Asian type (P2). Genetic diversity, principal component analysis (PCA), and population structure analyses revealed distinct accession clusters. Current results indicated that the proposed hybridization occurred widely between P1 and P2.
UNASSIGNED: Most of the accessions (80.99%) were of mixed ancestry, perhaps including multiple hybridization events and regional selection, which merits further investigation.
■对来自世界不同地区的284个芒果种质进行了高通量测序和特定基因座扩增片段(SLAF)文库构建,以产生基因组单核苷酸多态性(SNP)。
■过滤后,获得包含539.61M读数的原始数据。共检测到505,300个SLAF,其中,205,299是多态的。最后,29,136个SNP被用来剖析人口结构,遗传关系,和遗传多样性。284个芒果品种分为两大类:一组主要由澳大利亚的芒果品种组成,美国,古巴,印度,加勒比,以色列,巴基斯坦,几内亚,缅甸,中国,斯里兰卡,属于印度类型(P1);另一组包含来自菲律宾的芒果种质,泰国,印度尼西亚,越南,柬埔寨,马来西亚,新加坡,属于东南亚型(P2)。遗传多样性,主成分分析(PCA),人口结构分析揭示了不同的加入集群。当前结果表明,拟议的杂交广泛发生在P1和P2之间。
■大多数(80.99%)是混合血统,可能包括多个杂交事件和区域选择,值得进一步调查。