PDF(Pubmed)
Abstract：
Glanders caused by Burkholderia mallei is one of the most dangerous zoonotic diseases in solipeds. Clinical diagnosis of this disease in its early stages in horses, is difficult. This study investigated serological and molecular identification of B. mallei in East Azerbaijan province. In the third and fourth quarters of 2020, throughout 2021, and in the first and second quarters of 2022, the complement fixation test (CFT) was performed on 350 horses. The malleination was used to confirm the positive CFT cases. Blood samples were taken for culture and for preparing serums to perform the enzyme-linked immunosorbent assay (ELISA). Deep eye discharge, nostril, cutaneous ulcers and lymph fluid swabs were cultured, and polymerase chain reaction (PCR) was carried out. Eleven horses were CFT-positive. Based on the malleination on the 11 horses, six were affected by glanders, five were not affected (false positive), and one horse was CFT-negative despite exhibiting clinical signs. It was positive by malleination, ELISA and PCR. A total number of seven positive cases of glanders were diagnosed. The B. mallei could not be isolated, but the Burkholderia cepacia complex was isolated in one case. Except for three cases, the results of the CFT, mallein and ELISA tests were consistent. The amount of confidence interval was 95.00%. It is suggested that ELISA could be used as a complement to CFT in screening and, if positive results are observed in one of the tests, the entire herd must be examined more accurately using the mallein and western blot confirmatory tests.
摘要：
由Burkholderiamallei引起的Granders是solipes中最危险的人畜共患疾病之一。这种疾病在马早期的临床诊断，很难。这项研究调查了阿塞拜疆东部的B.mallei的血清学和分子鉴定。在2020年的第三和第四季度，整个2021年以及2022年的第一和第二季度，对350匹马进行了补充固定测试（CFT）。锤状用于确认CFT阳性病例。取血样用于培养和制备血清以进行酶联免疫吸附测定(ELISA)。眼睛深处的分泌物,鼻孔,培养皮肤溃疡和淋巴液拭子，并进行聚合酶链反应(PCR)。11匹马CFT阳性。根据11匹马的锤炼，六个受到腺体的影响，五个没有受到影响（假阳性），一匹马尽管表现出临床症状，但CFT阴性。通过锤炼是积极的，ELISA和PCR。共诊断出7例腺体阳性病例。B.Mallei无法被隔离,但在一个病例中，伯克霍尔德菌被分离。除了三个案例,CFT的结果，mallein和ELISA测试是一致的。置信区间为95.00%。建议ELISA可以作为CFT的补充，用于筛查，如果在其中一项测试中观察到阳性结果，必须使用mallein和westernblot确证试验更准确地检查整个牛群。
