PDF(Pubmed)
Abstract：
Hydrogels exhibit exceptional suitability as wound dressing due to their remarkable three-dimensional (3D) characteristics. Here, we have reported the fabrication of hydrogels from biopolymers carboxymethyl cellulose (CMC), polyvinyl alcohol (PVA), and gelatin via a simple blending method to mimic the natural extracellular matrix. Scanning electron microscopy (SEM), water contact meters (WCM), and Fourier-transform infrared spectroscopy (FTIR) were used to evaluate the chemical structural, morphological, and wettability behavior. The wetting and degradation behavior were also found to be different for different formulations (Min. (51.60o) and Max. (113.60o)) and (Min. (38.82 mg) and Max. (3.72 mg)), respectively. Swelling was investigated in different media, including phosphate buffer saline solution (PBS) and aqueous media. It was observed that the hydrogel displayed the highest degree of swelling in an aqueous medium (Min. (597.32-1121.49 %) and Max. (1089.51-2139.73 %)) compared to PBS media (Min. (567.01-1021.85 %) and Max. (899.13-1639.17 %)). The release of Neomycin was studied in a PBS medium via the Franz diffusion method at 37 °C. The maximal release in various media demonstrated pH-responsive behavior. The viability and proliferation of fibroblast (3T3) cell lines were examined in vitro to evaluate cytocompatibility. Human Embryonic Kidney (HEK) 293 cells were used to evaluate the hydrogels\' ability to promote vascularization and angiogenesis. Therefore, the data demonstrate that hydrogels that have been manufactured have qualities that make them promising for use as wound dressings in wound healing applications.
摘要：
水凝胶由于其显著的三维(3D)特性而表现出作为伤口敷料的优异适用性。这里,我们已经报道了由生物聚合物羧甲基纤维素（CMC）制造水凝胶，聚乙烯醇(PVA),和明胶通过简单的混合方法模拟天然细胞外基质。扫描电子显微镜（SEM），水接触仪表（WCM），和傅里叶变换红外光谱(FTIR)用于评估化学结构,形态学,和润湿性行为。还发现不同配方的润湿和降解行为不同（Min。（51.60o）和最大。（113.60o））和（最小值。（38.82毫克）和最大。（3.72毫克）），分别。肿胀在不同的媒体进行了调查，包括磷酸盐缓冲盐溶液(PBS)和水性介质。观察到水凝胶在水性介质中表现出最高的溶胀度(Min.（597.32-1121.49%）和最大。（1089.51-2139.73%））与PBS培养基（Min。（567.01-1021.85%）和最大。（899.13-1639.17%））。在PBS介质中通过Franz扩散方法在37°C下研究新霉素的释放。在各种介质中的最大释放表现出pH响应行为。体外检查成纤维细胞（3T3）细胞系的活力和增殖以评估细胞相容性。人胚胎肾(HEK)293细胞用于评价水凝胶促进血管形成和血管生成的能力。因此,数据表明,已经制造的水凝胶具有使它们有希望在伤口愈合应用中用作伤口敷料的品质。
