PDF(Pubmed)
Abstract：
UNASSIGNED: The purpose of this study was to determine what proteins are present in the ciliary body (CB). To accomplish this, we conducted a proteomic analysis of the CB of cynomolgus monkeys. We also determined the location of the proteins in CB by immunohistology.
UNASSIGNED: The eyes of euthanized cynomolgus monkeys were enucleated, and the CB, were isolated from the eyes. Proteins were extracted from the CB and determined by liquid chromatography-mass spectrometry. Separated CB epithelial cells were cultured, and the proteins expressed in the CB were determined by Western blotting. The location of these proteins in the CB was determined by immunohistochemical staining. We also investigated whether adding dexamethasone to the culture medium changed protein expression by the epithelial cells.
UNASSIGNED: Proteomic analysis of the CBs showed that 813 proteins were expressed in the epithelium and stroma. These proteins included the small guanosine triphosphate-binding protein Rab8 and the ezrin/radixin/moesin (ERM) family. Tissue and immunohistological staining confirmed the colocalization of these proteins in non-pigmented CB epithelium. Western blotting of cultured CB epithelial cell lysates showed a tendency that adding dexamethasone changed Rab8 protein expression levels.
UNASSIGNED: Proteomic analysis of CBs identified several proteins involved in the transport and secretion of proteins. These proteins may be involved in the production of aqueous humor and protein secretion by the CB.
UNASSIGNED: The eyes of euthanized cynomolgus monkeys were enucleated, and the CB, were isolated from the eyes. Proteins were extracted from the CB and determined by liquid chromatography-mass spectrometry. Separated CB epithelial cells were cultured, and the proteins expressed in the CB were determined by Western blotting. The location of these proteins in the CB was determined by immunohistochemical staining. We also investigated whether adding dexamethasone to the culture medium changed protein expression by the epithelial cells.
UNASSIGNED: Proteomic analysis of the CBs showed that 813 proteins were expressed in the epithelium and stroma. These proteins included the small guanosine triphosphate-binding protein Rab8 and the ezrin/radixin/moesin (ERM) family. Tissue and immunohistological staining confirmed the colocalization of these proteins in non-pigmented CB epithelium. Western blotting of cultured CB epithelial cell lysates showed a tendency that adding dexamethasone changed Rab8 protein expression levels.
UNASSIGNED: Proteomic analysis of CBs identified several proteins involved in the transport and secretion of proteins. These proteins may be involved in the production of aqueous humor and protein secretion by the CB.
摘要：
■这项研究的目的是确定睫状体（CB）中存在哪些蛋白质。要做到这一点，我们对食蟹猴的CB进行了蛋白质组学分析。我们还通过免疫组织学确定了蛋白质在CB中的位置。
■将安乐死的食蟹猴的眼睛摘除，和CB，与眼睛隔离。从CB中提取蛋白质并通过液相色谱-质谱法测定。培养分离的CB上皮细胞，用蛋白质印迹法测定CB中表达的蛋白质。通过免疫组织化学染色确定这些蛋白质在CB中的位置。我们还研究了向培养基中添加地塞米松是否会改变上皮细胞的蛋白质表达。
■CBs的蛋白质组学分析显示813种蛋白质在上皮和基质中表达。这些蛋白质包括小的三磷酸鸟苷结合蛋白Rab8和ezrin/radixin/moesin（ERM）家族。组织和免疫组织学染色证实了这些蛋白质在非色素CB上皮中的共定位。培养的CB上皮细胞裂解物的Western印迹显示出添加地塞米松改变Rab8蛋白表达水平的趋势。
■CBs的蛋白质组学分析鉴定了几种参与蛋白质运输和分泌的蛋白质。这些蛋白质可能参与房水的产生和CB的蛋白质分泌。
■将安乐死的食蟹猴的眼睛摘除，和CB，与眼睛隔离。从CB中提取蛋白质并通过液相色谱-质谱法测定。培养分离的CB上皮细胞，用蛋白质印迹法测定CB中表达的蛋白质。通过免疫组织化学染色确定这些蛋白质在CB中的位置。我们还研究了向培养基中添加地塞米松是否会改变上皮细胞的蛋白质表达。
■CBs的蛋白质组学分析显示813种蛋白质在上皮和基质中表达。这些蛋白质包括小的三磷酸鸟苷结合蛋白Rab8和ezrin/radixin/moesin（ERM）家族。组织和免疫组织学染色证实了这些蛋白质在非色素CB上皮中的共定位。培养的CB上皮细胞裂解物的Western印迹显示出添加地塞米松改变Rab8蛋白表达水平的趋势。
■CBs的蛋白质组学分析鉴定了几种参与蛋白质运输和分泌的蛋白质。这些蛋白质可能参与房水的产生和CB的蛋白质分泌。
