Abstract：
Enhancing endothelial cell growth on small-diameter vascular grafts produced from decellularized tissues or synthetic substrates is pivotal for preventing thrombosis. While optimized decellularization protocols can preserve the structure and many components of the extracellular matrix (ECM), the process can still lead to the loss of crucial basement membrane proteins, such as laminin, collagen IV, and perlecan, which are pivotal for endothelial cell adherence and functional growth. This loss can result in poor endothelialization and endothelial cell activation causing thrombosis and intimal hyperplasia. To address this, the basement membrane\'s ECM is emulated on fiber substrates, providing a more physiological environment for endothelial cells. Thus, fibroblasts are cultured on fiber substrates to produce an ECM membrane substrate (EMMS) with basement membrane proteins. The EMMS then underwent antigen removal (AR) treatment to eliminate antigens from the membrane while preserving essential proteins and producing an AR-treated membrane substrate (AMS). Subsequently, human endothelial cells cultured on the AMS exhibited superior proliferation, nitric oxide production, and increased expression of endothelial markers of quiescence/homeostasis, along with autophagy and antithrombotic factors, compared to those on the decellularized aortic tissue. This strategy showed the potential of pre-endowing fiber substrates with a basement membrane to enable better endothelization.
摘要：
增强由去细胞化组织或合成基质产生的小直径血管移植物上的内皮细胞生长对于预防血栓形成至关重要。虽然优化的去细胞化方案可以保留细胞外基质（ECM）的结构和许多组分，这个过程仍然会导致关键的基底膜蛋白质的丢失，比如层粘连蛋白，胶原蛋白IV，和Perlecan,这是内皮细胞粘附和功能生长的关键。这种损失可导致不良的内皮化和内皮细胞活化,引起血栓形成和内膜增生。为了解决这个问题，基底膜的ECM在纤维基质上模拟，为内皮细胞提供更多的生理环境。因此，在纤维基质上培养成纤维细胞以产生具有基底膜蛋白的ECM膜基质(EMMS)。然后对EMMS进行抗原去除(AR)处理以从膜中消除抗原,同时保留必需蛋白并产生AR处理的膜底物(AMS)。随后，在AMS上培养的人内皮细胞表现出优越的增殖，一氧化氮生产，和增加的内皮标志物的静止/体内平衡的表达，以及自噬和抗血栓因子，与去细胞化主动脉组织上的那些相比。该策略显示了预先赋予纤维底物基底膜以实现更好的内皮素的潜力。
