Abstract：
In mass disaster events, forensic DNA laboratories may be called upon to quickly pivot their operations toward identifying bodies and reuniting remains with family members. Ideally, laboratories have considered this possibility in advance and have a plan in place. Compared with traditional short tandem repeat (STR) typing, single nucleotide polymorphisms (SNPs) may be better suited to these disaster victim identification (DVI) scenarios due to their small genomic target size, resulting in an improved success rate in degraded DNA samples. As the landscape of technology has shifted toward DNA sequencing, many forensic laboratories now have benchtop instruments available for massively parallel sequencing (MPS), facilitating this operational pivot from routine forensic STR casework to DVI SNP typing. Herein, we present the commercially available SNP sequencing assays amenable to DVI, we use data simulations to explore the potential for kinship prediction from SNP panels of varying sizes, and we give an example DVI scenario as context for presenting the matrix of considerations: kinship predictive potential, cost, and throughput of current SNP assay options. This information is intended to assist laboratories in choosing a SNP system for disaster preparedness.
摘要：
在大规模灾难事件中，法医DNA实验室可能会被要求迅速将其操作转向识别尸体并与家庭成员团聚。理想情况下,实验室已经事先考虑了这种可能性，并制定了计划。与传统的短串联重复(STR)分型相比，单核苷酸多态性（SNP）可能更适合这些灾难受害者识别（DVI）场景，因为它们的基因组靶大小小，导致降解的DNA样品的成功率提高。随着技术领域转向DNA测序，许多法医实验室现在都有可用于大规模平行测序(MPS)的台式仪器，促进从常规法医STR案例工作到DVISNP分型的操作枢纽。在这里，我们提出了适用于DVI的市售SNP测序测定法，我们使用数据模拟来探索从不同大小的SNP面板中进行亲属关系预测的潜力，我们给出了一个示例DVI场景作为上下文来呈现考虑因素矩阵：亲属关系预测潜力，成本,和当前SNP测定选项的通量。此信息旨在帮助实验室选择用于备灾的SNP系统。
