Abstract：
The interactions among plant viruses, insect vectors, and host plants have been well studied; however, the roles of insect viruses in this system have largely been neglected. We investigated the effects of MpnDV infection on aphid and PVY transmission using bioassays, RNA interference (RNAi), and GC-MS methods and green peach aphid (Myzus persicae (Sulzer)), potato virus Y (PVY), and densovirus (Myzus persicae nicotianae densovirus, MpnDV) as model systems. MpnDV increased the activities of its host, promoting population dispersal and leading to significant proliferation in tobacco plants by significantly enhancing the titer of the sesquiterpene (E)-β-farnesene (EβF) via up-regulation of expression levels of the MpFPPS1 gene. The proliferation and dispersal of MpnDV-positive individuals were faster than that of MpnDV-negative individuals in PVY-infected tobacco plants, which promoted the transmission of PVY. These results combined showed that an insect virus may facilitate the transmission of a plant virus by enhancing the locomotor activity and population proliferation of insect vectors. These findings provide novel opportunities for controlling insect vectors and plant viruses, which can be used in the development of novel management strategies.
摘要：
植物病毒之间的相互作用，昆虫媒介,和寄主植物已经得到了很好的研究；然而，昆虫病毒在这个系统中的作用在很大程度上被忽视了。我们使用生物测定法研究了MpnDV感染对蚜虫和PVY传播的影响，RNA干扰(RNAi),和GC-MS方法和绿桃蚜虫（Myzuspersicae（Sulzer）），马铃薯Y病毒(PVY),和densovirus(Myzuspicaenicotianaedensovirus,MpnDV)作为模型系统。MpnDV增加了宿主的活动，通过上调MpFPPS1基因的表达水平，显着提高倍半萜（E）-β-法尼烯（EβF）的滴度，从而促进种群扩散并导致烟草植物的显着增殖。在PVY感染的烟草植株中，MpnDV阳性个体的增殖和扩散快于MpnDV阴性个体，促进了PVY的传播。这些结果结合显示,昆虫病毒可以通过增强昆虫载体的运动活性和种群增殖来促进植物病毒的传播。这些发现为控制昆虫媒介和植物病毒提供了新的机会，可用于开发新的管理策略。
