Abstract:
Aquilaria yunnanensis is an endangered agarwood-producing tree currently listed on the IUCN Red List of Threatened Species. The agarwood it produces has important medicinal and economic value, but its population has sharply declined due to human destruction and habitat reduction. Therefore, obtaining genomic information on A. yunnanensis is beneficial for its protection work. We assembled a chromosome-level reference genome of A. yunnanensis by using BGI short reads, PacBio HiFi long reads, coupled with Hi-C technology. The final genome assembly of A. yunnanensis is 847.04 Mb, with N50 size of 99.68 Mb, in which 805.49 Mb of the bases were anchored on eight pseudo-chromosomes. Two gapless pseudo-chromosomes were detected in the assembly. A total of 27,955 protein-coding genes as well as 74.65% repetitive elements were annotated. These findings may provide valuable resources in conservation, functional genomics, and molecular breeding of A. yunnanensis, as well as the molecular phylogenetics and evolutionary patterns in Aquilaria.
摘要:
云南沉香是一种濒临灭绝的沉香树,目前已被列入IUCN濒危物种红色名录。其生产的沉香具有重要的药用和经济价值,但是由于人类的破坏和栖息地的减少,其人口急剧下降。因此,获得云南A的基因组信息有利于其保护工作。我们使用BGI短读数组装了云南A的染色体水平参考基因组,PacBioHiFi长读到,再加上Hi-C技术。云南A.的最终基因组组装为847.04Mb,N50大小为99.68Mb,其中805.49Mb的碱基锚定在八个假染色体上。在组装中检测到两个无间隙假染色体。总共注释了27,955个蛋白质编码基因以及74.65%的重复元件。这些发现可能为保护提供宝贵的资源,功能基因组学,和云南A的分子育种,以及沉香的分子系统发育和进化模式。