Abstract：
Pressure ulcers, or bedsores, are created by areas of the skin under prolonged pressure and can lead to skin and underlying tissue damage. The present study evaluated the effects of carboxymethyl cellulose/sodium alginate/gelatin (CMC/Alg/Gel) hydrogel containing doxycycline (DOX) on improving the healing process of pressure ulcers. The magnet was used to apply pressure on the dorsum skin rat to induce a pressure ulcer model. Then sterile gauze, CMC/Alg/Gel, and CMC/Alg/Gel/1% w/v DOX hydrogels were used to cover the wounds. Blood compatibility, weight loss, cytocompatibility, drug release rate, cell viability, wound closure, and re-epithelialization were evaluated in all animals on the 14th day after treatment. In vivo results and histopathological evaluation showed 56.66% wound closure and the highest re-epithelialization in the CMC/Alg/Gel/1% w/v DOX hydrogel group (14 days after treatment). Furthermore, real-time PCR results indicated that the hydrogel containing DOX significantly decreased the expression of the MMP family consisting of MMP2 and MMP9 mRNA and also increased the expression of vascular endothelial growth factor VEGF mRNA. This study suggested that the addition of DOX, an antibiotic and MMP inhibitor, to hydrogels may be effective in the healing process of pressure ulcers.
摘要：
压疮,或者褥疮,由长期压力下的皮肤区域产生，并可能导致皮肤和下面的组织损伤。本研究评估了含有多西环素（DOX）的羧甲基纤维素/海藻酸钠/明胶（CMC/Alg/Gel）水凝胶在改善压疮愈合过程中的作用。用磁铁对大鼠背部皮肤施加压力，诱导压疮模型。然后是无菌纱布,CMC/Alg/Gel,和CMC/Alg/Gel/1%w/vDOX水凝胶用于覆盖伤口。血液相容性,减肥,细胞相容性,药物释放率，细胞活力，伤口闭合,并在治疗后第14天评估所有动物的上皮再形成。体内结果和组织病理学评估显示,在CMC/Alg/Gel/1%w/vDOX水凝胶组中(治疗后14天),56.66%的伤口闭合和最高的再上皮化。此外，real-timePCR结果表明，含有DOX的水凝胶显着降低了由MMP2和MMP9mRNA组成的MMP家族的表达，并增加了血管内皮生长因子VEGFmRNA的表达。这项研究表明，添加DOX，抗生素和MMP抑制剂，水凝胶在压疮的愈合过程中可能是有效的。
