关键词: Adeno-associated virus Analytical ultracentrifugation High rotor speeds High-resolution Interference detection Low temperature Nanoparticles Sedimentation velocity Size distribution Viral vectors

Mesh : Dependovirus / genetics isolation & purification Ultracentrifugation / methods Humans

来  源:   DOI:10.1016/j.ab.2024.115617

Abstract:
Data are presented demonstrating that absorbance detection can be used during high-speed sedimentation velocity analytical ultracentrifugation (hs-SV-AUC) experiments to characterize the size distribution of adeno-associated virus (AAV) drug products accurately. Advantages and limitations of being able to use this detector in this specific type of SV-AUC experiment are discussed.
摘要:
所提供的数据表明,在高速沉降速度分析超速离心(hs-SV-AUC)实验中可以使用吸光度检测来准确表征腺相关病毒(AAV)药物产品的大小分布。讨论了能够在这种特定类型的SV-AUC实验中使用该检测器的优点和局限性。
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