Abstract：
Total antioxidants play a crucial role in human health, and detection of the total antioxidant capacity (TAC) has broad application prospects in fields such as food safety, environmental assessment, and disease diagnosis. However, a long detection time, cumbersome steps, high cost, reliance on professional equipment, and nonportability still remain significant challenges. In this work, an efficient strategy of point-of-care testing (POCT) of the TAC in body fluids by nanozyme-catalyzed colorimetric paper-based microfluidic sensors is proposed. The paper-based microfluidic sensors coupled with a smartphone can reduce testing costs and provide portability. The nanozyme prepared by the solvothermal method presents Michaelis constants of 0.11 and 0.129 mM for H2O2 and TMB, respectively. A method for immobilizing nanozymes and chromogenic agents on a paper-based microfluidic chip is established. Based on smartphone photography and image grayscale extraction, the TAC can be qualitatively detected with a detection limit and linear range of 33.4 and 50-700 μM, respectively. Furthermore, the proposed sensor can realize the one-step quantitative analysis of the TAC in body fluids (blood, saliva, and sweat) within 15 min. The proposed nanozyme-catalyzed colorimetric paper-based microfluidic sensors presented in this study exhibit promising application prospects in the fields of biochemical analysis and POCT.
摘要：
总抗氧化剂在人类健康中起着至关重要的作用，总抗氧化能力(TAC)的检测在食品安全等领域具有广阔的应用前景,环境评估,和疾病诊断。然而,很长的检测时间，繁琐的步骤，高成本,依赖专业设备，和不可携带仍然是重大挑战。在这项工作中,提出了一种通过纳米酶催化比色纸基微流控传感器对体液中TAC进行即时检测（POCT）的有效策略。与智能手机耦合的基于纸的微流体传感器可以降低测试成本并提供便携性。溶剂热法制备的纳米酶对H2O2和TMB的米氏常数为0.11和0.129mM，分别。建立了一种在纸基微流控芯片上固定纳米酶和显色剂的方法。基于智能手机摄影和图像灰度提取,TAC可以定性检测，检测限和线性范围为33.4和50-700μM，分别。此外，所提出的传感器可以实现对体液中TAC(血液，唾液,和汗水）在15分钟内。本研究中提出的纳米酶催化比色纸基微流控传感器在生化分析和POCT领域具有广阔的应用前景。
