Abstract：
The hybridoma method for production of monoclonal antibodies has been a cornerstone of biomedical research for several decades. Here we convert the monoclonal antibody sequence from mouse-derived hybridomas into a \"devilized\" recombinant antibody with devil IgG heavy chain and IgK light chain. The chimeric recombinant antibody can be used in functional assays, immunotherapy, and to improve understanding of antibodies and Fc receptors in Tasmanian devils. The process can be readily modified for other species.
摘要：
用于产生单克隆抗体的杂交瘤方法几十年来一直是生物医学研究的基石。在这里，我们将来自小鼠来源的杂交瘤的单克隆抗体序列转化为具有魔鬼IgG重链和IgK轻链的“变性”重组抗体。嵌合重组抗体可用于功能测定，免疫疗法，并提高对塔斯马尼亚恶魔抗体和Fc受体的了解。该方法可以容易地针对其他物种进行修改。
