Abstract：
Immunofluorescence microscopy is a powerful technique using fluorescently labelled antibodies which can be used to visualize proteins in the nucleus. A key advantage of this method is that it can provide insight into the spatial organization and the localization of nuclear proteins, which can provide elucidation of their function. Here, we provide a protocol for immunofluorescence staining in the nucleus, which has successfully been used to visualize histone modifications and nuclear bodies in human and mouse B lymphocytes, using as few as 1 × 104-5 × 104 cells.
摘要：
免疫荧光显微镜是一种使用荧光标记抗体的强大技术，可用于可视化细胞核中的蛋白质。这种方法的一个关键优点是它可以提供对空间组织和核蛋白定位的洞察力，可以阐明它们的功能。这里,我们提供了细胞核免疫荧光染色的方案，已成功用于可视化人和小鼠B淋巴细胞中的组蛋白修饰和核体，使用少至1×104-5×104个细胞。
