PDF(Pubmed)
Abstract：
We demonstrate that the attachment of 30-170 bp dsDNA oligomers to ssDNA viral genomes gives a significant additional mobility shift in micelle-tagging electrophoresis (MTE). In MTE, a modified peptide nucleic acid amphiphile is attached to the viral genome to bind drag-inducing micelles present in capillary electrophoresis running buffers. Further attachment of 30-170 bp dsDNA oligomers drastically shifts the mobility of the 5.1 kB ssDNA genome of mouse minute virus (MMV), providing a new mechanism to improve resolution in CE-based analysis of kilobase nucleic acids. A model based on biased-reptation electrophoresis, end-labeled free-solution electrophoresis, and Ferguson gel-filtration theory is presented to describe the observed mobility shifts.
摘要：
我们证明了30-170bpdsDNA寡聚物与ssDNA病毒基因组的连接在胶束标记电泳（MTE）中产生了显着的额外迁移率变化。在MTE中,修饰的肽核酸两亲物附着于病毒基因组以结合存在于毛细管电泳运行缓冲液中的药物诱导胶束。30-170bpdsDNA寡聚物的进一步连接极大地改变了小鼠微小病毒（MMV）的5.1kBssDNA基因组的迁移率，提供了一种新的机制来提高基于CE的千碱基核酸分析的分辨率。基于偏向重复电泳的模型，端标自由溶液电泳,并提出了Ferguson凝胶过滤理论来描述观察到的迁移率变化。
