Abstract：
Environmental carcinogens exert their carcinogenic effects by forming DNA adducts. This type of DNA damage can also be formed endogenously as a result of, e.g., oxidative damage. Unrepaired DNA adducts may induce mutations in critical genes, leading to the initiation of chemical carcinogenesis. Therefore, detection, identification, and quantification of DNA adducts is essential for cancer risk assessment. Over the last 50 years, the major DNA adducts formed by different classes of environmental carcinogens were characterized. With the development of techniques such as 32P-postlabeling, their measurement was implemented into molecular epidemiology. Advances in liquid chromatography-tandem mass spectrometry (LC-MS ) made the measurement of adducts more precise and allowed to gain knowledge about their identity and structures. Therefore, opened the way to DNA adductomics, the \"omics\" approach investigating DNA adducts comprehensively, similarly to proteomics. This review presents the historical perspective of DNA adducts research and the emerging field of adductomics.
摘要：
环境致癌物通过形成DNA加合物来发挥其致癌作用。这种类型的DNA损伤也可以是内源性的，例如，氧化损伤。未修复的DNA加合物可能会诱导关键基因的突变，导致化学致癌作用的开始。因此,检测,identification,DNA加合物的定量对于癌症风险评估至关重要。在过去的50年里,表征了由不同类别的环境致癌物形成的主要DNA加合物。随着32P-postlabel等技术的发展,他们的测量被实施为分子流行病学。液相色谱-串联质谱（LC-MS）的进步使加合物的测量更加精确，并允许获得有关其身份和结构的知识。因此,开辟了DNA内加酶学的道路，“组学”方法全面调查DNA加合物，类似于蛋白质组学。这篇综述介绍了DNA加合物研究的历史观点和内加学的新兴领域。
