Abstract：
The performance and long-term durability of dental implants hinge on the quality of bone integration and their resistance to bacteria. This research aims to introduce a surface modification strategy for zirconia implants utilizing femtosecond laser ablation techniques, exploring their impact on osteoblast cell behavior and bacterial performance, as well as the integral factors influencing the soft tissue quality surrounding dental implants. Ultrafast lasers were employed to craft nanoscale groove geometries on zirconia surfaces, with thorough analyses conducted using x-ray diffraction, scanning electron microscopy, atomic force microscopy, and water contact angle measurements. The study evaluated the response of human fetal osteoblastic cell lines to textured zirconia ceramics by assessing alkaline phosphatase activity, collagen I, and interleukin 1βsecretion over a 7 day period. Additionally, the antibacterial behavior of the textured surfaces was investigated usingFusobacterium nucleatum, a common culprit in infections associated with dental implants. Ciprofloxacin (CIP), a widely used antibacterial antibiotic, was loaded onto zirconia ceramic surfaces. The results of this study unveiled a substantial reduction in bacterial adhesion on textured zirconia surfaces. The fine biocompatibility of these surfaces was confirmed through the MTT assay and observations of cell morphology. Moreover, the human fetal osteoblastic cell line exhibited extensive spreading and secreted elevated levels of collagen I and interleukin 1βin the modified samples. Drug release evaluations demonstrated sustained CIP release through a diffusion mechanism, showcasing excellent antibacterial activity against pathogenic bacteria, includingStreptococcus mutans, Pseudomonas aeruginosa, andEscherichia coli.
摘要：
牙种植体的性能和长期耐久性取决于骨整合的质量及其对细菌的抵抗力。本研究旨在介绍一种利用飞秒激光烧蚀技术的氧化锆植入物的表面改性策略，探索它们对成骨细胞行为和细菌性能的影响，以及影响种植体周围软组织质量的综合因素。使用超快激光器在氧化锆表面上制作纳米级凹槽几何形状，通过使用X射线衍射进行彻底的分析，扫描电子显微镜，原子力显微镜,和水接触角测量。该研究通过评估碱性磷酸酶活性来评估人胎成骨细胞系对纹理氧化锆陶瓷的反应，胶原蛋白I，和白细胞介素1β分泌超过7天。此外,使用具核梭菌研究了纹理表面的抗菌行为，与种植牙相关的感染的常见元凶。环丙沙星(CIP),一种广泛使用的抗菌抗生素，加载到氧化锆陶瓷表面。这项研究的结果揭示了细菌在纹理氧化锆表面上的粘附的显著减少。通过MTT测定和细胞形态观察证实了这些表面的良好生物相容性。此外，在改良样品中，人胎儿成骨细胞系表现出广泛的扩散和分泌的I型胶原和白介素1β水平升高。药物释放评价表明CIP通过扩散机制持续释放，对病原菌表现出优异的抗菌活性，包括变形链球菌，铜绿假单胞菌,和大肠杆菌。
