PDF(Pubmed)
Abstract：
UNASSIGNED: Campylobacter spp. are a public health concern, yet there is still no effective vaccine or medicine available.
UNASSIGNED: Here, we developed a Campylobacter jejuni-specific antibody and found that it targeted a menaquinol cytochrome c reductase complex QcrC.
UNASSIGNED: The antibody was specifically reactive to multiple C. jejuni strains including clinical isolates from patients with acute enteritis and was found to inhibit the energy metabolism and growth of C. jejuni. Different culture conditions produced different expression levels of QcrC in C. jejuni, and these levels were closely related not only to the energy metabolism of C. jejuni but also its pathogenicity. Furthermore, immunization of mice with recombinant QcrC induced protective immunity against C. jejuni infection.
UNASSIGNED: Taken together, our present findings highlight a possible antibody- or vaccination-based strategy to prevent or control Campylobacter infection by targeting the QcrC-mediated metabolic pathway.
UNASSIGNED: Here, we developed a Campylobacter jejuni-specific antibody and found that it targeted a menaquinol cytochrome c reductase complex QcrC.
UNASSIGNED: The antibody was specifically reactive to multiple C. jejuni strains including clinical isolates from patients with acute enteritis and was found to inhibit the energy metabolism and growth of C. jejuni. Different culture conditions produced different expression levels of QcrC in C. jejuni, and these levels were closely related not only to the energy metabolism of C. jejuni but also its pathogenicity. Furthermore, immunization of mice with recombinant QcrC induced protective immunity against C. jejuni infection.
UNASSIGNED: Taken together, our present findings highlight a possible antibody- or vaccination-based strategy to prevent or control Campylobacter infection by targeting the QcrC-mediated metabolic pathway.
摘要：
■弯曲杆菌属。是一个公共卫生问题，然而，仍然没有有效的疫苗或药物可用。
■这里，我们开发了一种空肠弯曲杆菌特异性抗体，并发现它靶向甲萘酚细胞色素c还原酶复合物QcrC。
■该抗体对包括来自急性肠炎患者的临床分离株在内的多种空肠杆菌菌株具有特异性反应性,并且发现该抗体抑制空肠杆菌的能量代谢和生长。不同的培养条件在空肠弯曲菌中产生不同的QcrC表达水平,这些水平不仅与空肠弯曲菌的能量代谢密切相关，而且与其致病性密切相关。此外，用重组QcrC免疫小鼠诱导针对空肠弯曲杆菌感染的保护性免疫。
■放在一起，我们目前的研究结果强调了一种可能的基于抗体或疫苗接种的策略,通过靶向QcrC介导的代谢途径来预防或控制弯曲杆菌感染.
■这里，我们开发了一种空肠弯曲杆菌特异性抗体，并发现它靶向甲萘酚细胞色素c还原酶复合物QcrC。
■该抗体对包括来自急性肠炎患者的临床分离株在内的多种空肠杆菌菌株具有特异性反应性,并且发现该抗体抑制空肠杆菌的能量代谢和生长。不同的培养条件在空肠弯曲菌中产生不同的QcrC表达水平,这些水平不仅与空肠弯曲菌的能量代谢密切相关，而且与其致病性密切相关。此外，用重组QcrC免疫小鼠诱导针对空肠弯曲杆菌感染的保护性免疫。
■放在一起，我们目前的研究结果强调了一种可能的基于抗体或疫苗接种的策略,通过靶向QcrC介导的代谢途径来预防或控制弯曲杆菌感染.
